RNA Interference Core

描述(由申请人提供)：该项目将满足INIA-WEST验证INIA候选基因在过度饮酒中的作用的需求。RNA干扰核心将为INIA-WEST研究人员提供RNA干扰介导的候选基因下调或过度表达的病毒载体。该项目的具体目的是设计针对感兴趣基因的短发夹状RNA(ShRNAs)并进行体外筛选，生产在特定脑区或转基因动物中用于基因沉默的病毒表达shRNAs，生产用于过表达感兴趣基因的病毒，以及验证基因沉默或在体内过度表达的有效性。INIA-West RNA干扰核心开发的病毒工具对于了解特定基因在调节过度饮酒中的作用至关重要。

DESCRIPTION (provided by applicant): This project will address the need of INIA-West for validating the role of INIA candidate genes in excessive drinking. The RNA Interference Core will provide viral vectors for RNA interference-mediated knockdown or over-expression of candidate genes to INIA-West Investigators. The specific aims of the project are to design short hairpin RNAs (shRNAs) targeting genes of interest and perform in vitro screening of shRNA efficacy, to produce virus expressing shRNAs for gene silencing in specific brain regions or in transgenic animals, to produce virus for over-expression of genes of interest, and to validate the effectiveness of gene silencing or over-expression in vivo. The viral tools developed by the INIA-West RNA Interference Core are crucial to understanding the role of specific genes in regulating excessive alcohol consumption.