National Center for Functional Glycomics

描述(由申请人提供)： 在这里，我们建议建立一个“国家功能糖链中心”(NCFG)，专注于五个主要目标：技术研发(TR&D)；推动与研发项目相关的生物医学项目(DBP)；协作服务；培训；以及技术和研究发现的传播。这一应用程序的开发是由埃默里大学医学院糖链中心提供的非常成功的领导的结果，该中心拥有成功的功能性糖链联盟(CFG)的蛋白质-多糖相互作用核心(核心H)，这是2001-2011年间由NIGMS资助的独特资源，已被全球数百名研究人员使用。该中心对CFG定义的葡聚糖微阵列的应用，在过去的8年里已经收到了800份分析请求，使蛋白质-多糖相互作用的研究发生了革命性的变化。已定义的葡聚糖微阵列已经成为研究凝集素、抗体和病毒的糖结合特异性的基准，在这种奖励应用中，这些凝集素被称为糖结合蛋白(GBP)。固定化葡聚糖在研究GBP和功能糖组学方面的价值可以从该中心与世界各地600多名首席研究人员建立的合作关系中得到证明，在过去8年中，&gt；350篇同行评议的论文发表了。在过去的几年里，埃默里糖学中心的成员，他们是这项拟议的NCFG的首席研究员，在开发研究细胞糖功能和血糖分析的技术方面提供了创新的领导。在为这次NCFG提出的3个研发项目中，我们将1)扩大糖链微阵列技术，2)开发“鸟枪式糖链”作为研究天然细胞衍生的糖链识别的通用方法，3)开发不同的糖链展示技术，以及细胞头像-代表细胞糖链的独特呈现的颗粒。DBPs包括以下方面的研究：多糖在微生物和病毒感染中的作用，多糖和GBP在细胞黏附中的作用，先天和获得性免疫反应，以及有助于GBP相互作用的糖萼内的识别因子。NCFG将利用这些进展来推动强大的协作服务组成部分，以帮助生物医学研究社区使用和开发多糖微阵列和其他探索多糖识别的方法。我们将提供强有力的培训计划，并启动多个场所和战略，以传播NCFG的技术和科学发展。

DESCRIPTION (provided by applicant): Here we propose to establish a "National Center for Functional Glycomics" (NCFG) that is focused on 5 major objectives; Technology Research and Development (TR&D); Driving Biomedical Projects (DBPs) tied to TR&D Projects; Collaborative Services; Training; and Dissemination of Technologies and Research Discoveries. The development of this application is an outgrowth of the very successful leadership provided by the Glycomics Center at Emory University School of Medicine, which housed the Protein-Glycan Interaction Core (Core H) of the successful Consortium for Functional Glycomics (CFG), a unique NIGMS-funded resource from 2001-2011 that has been utilized by hundreds of investigators worldwide. The Center's applications of the CFG's Defined Glycan Microarray, which has received >800 requests for analyses in the past 8 years, has revolutionized studies on protein-glycan interactions. Defined glycan microarrays have become the benchmark in studying the glycan-binding specificity of lectins, antibodies, and viruses, which for this grant application are termed glycan-binding proteins (GBPs). The value of immobilized glycans in studying GBPs and functional glycomics is evidenced by the collaborations already established in the Center with over 600 Principal Investigators worldwide, resulting in >350 peer-reviewed publications in the past 8 years. Over the past several years, the members of the Glycomics Center at Emory, who are the lead investigators in this proposed NCFG, have provided innovative leadership in developing technologies for studying the functions of cellular glycomes and for glycomic analyses. In the 3 TR&D Projects proposed for this NCFG, we will 1) expand glycan microarray technologies, 2) develop "Shotgun Glycomics" as a general method for studying natural cell-derived glycan recognition, and 3) develop different glycan display technologies, as well as cellular avatars - particles that represent cellular glycomes in unique presentations. The DBPs include studies on the roles of glycans in microbial and viral infections, glycans and GBPs in cell adhesion, innate and adaptive immune responses, and recognition factors within the glycocalyx that contribute to GBP interactions. The NCFG will use these advances to drive a strong Collaborative Service component to aid the biomedical research community in the using and developing of glycan microarrays and other approaches to explore glycan recognition. We will offer a robust Training program, as well as initiate multiple venues and strategies to Disseminate the technological and scientific developments in the NCFG.