Immunohistochem & Molec Pathol

免疫组织化学和分子病理学核心（核心）的使命是促进教育，发展和免疫组织化学，免疫荧光和分子病理学工具的应用，为加州大学旧金山分校海伦迪勒家庭综合癌症中心（中心）成员。 核心将： 1.与中心成员合作，开发基于免疫组织化学（IHC）、免疫荧光（IMF）和原位杂交（FISH）的新抗体和遗传位点检测。 2.对动物和人体组织切片和细胞系进行常规免疫组织化学分析。 3.对中心的组织核心产生的组织阵列进行IHC和IMF分析。 4.对细胞系、动物模型和人类肿瘤中的DNA拷贝数进行FISH分析。 5.与中心成员合作进行显微切割，并从肿瘤切片中提取DNA和RNA。 6.对人类和小鼠DNA克隆的中期染色体进行基因组作图 该核心将与其他核心（特别是组织，基因组分析和细胞分析实验室核心）密切合作，使研究方案能够利用每个核心提供的资源和专业知识。

The Mission of the Immunohistochemistry and Molecular Pathology Core (the Core) is to facilitate education, development, and application of immunohistochemistry, immunofluorescence, and molecular pathology tools for the UCSF Helen Diller Family Comprehensive Cancer Center (Center) members. The Core will: 1. Develop immunohistochemistry (IHC), immunofluorescence (IMF) and in situ hybridization (FISH) based assays for new antibodies and genetic loci in collaboration with Center members. 2. Perform routine immunohistochemical analyses of animal and human tissue sections and cell lines. 3. Perform IHC and IMF analysis of tissue arrays produced by the Center's Tissue Core. 4. Perform FISH analyses for DNA copy number in cell lines, animal models, and human tumors. 5. Collaborate with Center members in microdissection, and extraction of DNA and RNA from tumor sections. 6. Perform genomic mapping onto metaphase chromosomes of human and mouse DNA clones The Core will work closely with other Cores (particularly the Tissue, Genome Analysis, and the Laboratory for Cell Analysis Cores) to allow research protocols to take advantage of the resources and expertise offered within each Core.