Single-Molecule Studies of Human RECQ5 Helicase Mechanisms

2012年10月29日，纽约大学朗格尼医学中心经历了一场前所未有的风暴，造成了破坏。 这深深地影响了我们的病人护理、研究和教育设施。有三种基本的科学 建筑物，Skirball研究所，Smilow研究中心和医学科学大楼（MSB），所有 这对机械，电气和管道系统造成了广泛的破坏，需要临时的 关闭我们的主校区由于电力中断和冷冻机故障，大量冷藏 冷冻的生物标本和试剂都丢失了Skirball研究所和Smilow研究中心 11月12日开始运作;然而，MSB遭受了最广泛的破坏，并且仍然关闭， 维修工作正在进行。 我的实验室位于医学科学大楼（MSB）。在飓风之前，我们建立了许多 分析，并获得了该项目的大部分试剂。由于飓风桑迪，我们失去了所有的 试剂，包括重组蛋白、质粒、FRET寡核苷酸、活性染料和专用化学品。 我们的仪器和工作的独特要求阻止了我们的全面搬迁，相反，我们保留了 临时空间，研究能力有限，不足以进行此处所述的工作。 因此，在桑迪之后的这段时间里，我们无法重新开始，再生或以其他方式继续我们的事业。 research.我们希望在2013年夏末/初秋之前重新填充我们的实验室， 在接下来的两个月里，功能。由于我们失去了所有的试剂，我们将不得不开始这个项目 重新这里提出的工作将使我们能够重新启动该项目，将其恢复到飓风前的状态， 允许我们继续并为竞争性NIH RO 1提案奠定基础。我们强调， 这里所描述的和拟议的时间轴将完成后，我们的实验室将再次投入使用。 这里描述的所有前期工作和所有试剂都需要再生。

NYU Langone Medical Center experienced an unprecedented storm on October 29th, 2012, causing damage that deeply impacted our patient care, research, and educational facilities. There are three basic science buildings, the Skirball Institute, the Smilow Research Center, and the Medical Science Building (MSB), all of which incurred extensive damage to mechanical, electrical, and plumbing systems, requiring the temporary closure of our main campus. Due to disruptions in power and freezers failing, a large number of refrigerated and frozen biospecimens and reagents were lost. The Skirball Institute and the Smilow Research Center were operational by November 12th; however, MSB sustained the most extensive damage, and remains closed as repairs are ongoing. My lab is located at the Medical Science Building (MSB). Prior to the hurricane we established many of the assays and obtained the majority of reagents for this project. As a result of hurricane Sandy, we lost all our reagents, including recombinant proteins, plasmids, FRET oligos, reactive dyes and specialized chemicals. The unique requirements of our instrumentation and work prevented our full relocation, and instead we retain temporary space with limited research capabilities that are insufficient for carrying the work described here. Consequently, in the interim since Sandy we could not reinitiate, regenerate or otherwise proceed in our research. We expect to repopulate our lab by the end of summer/early fall of 2013, and gradually regain full functionality in the two months that follow. Since we lost all of our reagents, we will have to start this project anew. The work proposed here will enable us reinitiate the project, restore it to its pre-hurricane state, and allow us to proceed and establish a basis for a competitive NIH RO1 proposal. We emphasize that all the work described here and the proposed timeline will be accomplished only after our lab will become operational again. All the preliminary work described here, and all the reagents will need to be regenerated.