120kV Transmission Electron Microscope for a Multi-user Microscopy Facility

描述（由申请人提供）：高分辨率形态学分析是生理和病理条件下生物学研究的组成部分。在细胞运动或细胞分泌过程中诱导的精细结构修饰通过透射电子显微镜（TEM）分析以高分辨率捕获。类似地，在各种实验条件下在亚细胞细胞器（晚期内体和溶酶体）中观察到的形态学变化可以通过TEM分析有力地可视化。细菌和真菌的分析可以提供非常有用的信息，它们在不同的实验条件下的形态变化。这是一个最先进的高分辨率透射电子显微镜和相关的数码相机的资金重新提交申请。该应用程序的主要用户所要求的TEM将是分析成像设备（AIF）的一部分，AIF是一种多用户成像资源，可供所有经过培训的研究者使用。目前，该设施中有一台1982年安装的老化JEOL JEM-100CX TEM，无法满足这些调查人员的需求。该应用中主要用户的共同科学需求是能够在不同实验条件下获得细胞、细胞器或病原体的高分辨率成像。一个新的TEM与高亮度LaB 6电子源和CCD相机将使高分辨率成像和数字数据记录。本提案中描述的透射电子显微镜将允许组装的主要用户分析病原体形态;内体生物发生和蛋白质分布的精细结构方面;以及否则将被排除的颗粒分泌。

DESCRIPTION (provided by applicant): High resolution morphological analysis is an integral part of biological investigations under both physiological and pathological conditions. The fine structure modifications induced during cell motility or cellular secretion are captured in high resolution by transmission electron microscopy (TEM) analysis. Similarly, morphological changes observed in sub-cellular organelles (late endosomes and lysosomes) during various experimental conditions can be powerfully visualized by TEM analysis. Analysis of bacteria and fungi can provide very useful information on their morphological changes under differential experimental conditions. This is a resubmission application for funding of a state of the art high resolution transmission electron microscope and an associated digital camera. The TEM the Major Users of this application are requesting will be part of the Analytical Imaging Facility (AIF, a multi user imaging resource, and available to all trained investigators. Currently, an ageing JEOL JEM-100CX TEM installed 1982 is present in the facility which does not fulfill the needs of these investigators. The common scientific need of the major user in this application is the ability to obtain high resolution imaging of cells, organelles or pathogens under different experimental conditions. A new TEM with a high brightness LaB6 electron source and CCD camera will enable high resolution imaging and digital data recording. The transmission electron microscope described in this proposal will allow the assembled Major Users to analyze pathogen morphology; fine structural aspect of endosomal biogenesis and protein distribution; and granule secretion which will be otherwise precluded.