A novel cytosolic chaperone complex in polyomavirus ER membrane transport

 描述（由申请人提供）：本申请的目的是阐明新型胞质伴侣复合物如何促进无包膜病毒多瘤病毒（Py）的内质网（ER）膜渗透。为了引起感染，Py与质膜上的宿主糖脂受体结合，被内吞，并运输到ER腔。在此，病毒颗粒经历构象变化以穿透ER膜并到达胞质溶胶。病毒从细胞质转运到细胞核，在那里病毒基因组的转录和复制随之发生，导致裂解性感染或细胞转化。Py如何从ER进入胞质溶胶是一个关键但知之甚少的事件。特别神秘的是调节这种ER膜渗透过程的胞质事件。因此，本申请集中于阐明所谓的BHS细胞溶质伴侣复合物在将Py从ER喷射到细胞溶质中的作用。

 DESCRIPTION (provided by applicant): The objective of this application is to clarify how a novel cytosolic chaperone complex promotes endoplasmic reticulum (ER) membrane penetration of the non-enveloped virus polyomavirus (Py). To cause infection, Py engages a host glycolipid receptor on the plasma membrane, becomes endocytosed, and traffics to the ER lumen. Here the viral particle undergoes conformational changes to penetrate the ER membrane and reach the cytosol. From the cytosol, the virus transports to the nucleus where transcription and replication of the viral genome ensue, leading to lytic infection or cellular transformation. How Py enters the cytosol from the ER is a crucial yet poorly understood event. Particularly enigmatic are cytosolic events that regulate this ER membrane penetration process. Accordingly this application is focused on elucidating the role of the so-called BHS cytosolic chaperone complex in ejecting Py into the cytosol from the ER.