Electron Microscopy Core

在过去的一年里，NHLBI电子显微镜核心设施支持了使用以下技术的项目： 1.组织细胞的固定、包埋、超薄切片和透射式EM数字成像。 2.EM免疫细胞化学，包括通过预包埋和冷冻金技术对细胞内和细胞表面的蛋白质和其他抗原进行免疫金定位。 3.大的蛋白质、聚合物和超分子结构以及脂质和膜小泡在透射电子显微镜成像中的阴性染色以及大分子的铂阴影。 4.小型生物、器官、组织和细胞以及人造基质等其他材料的固定、临界点干燥、溅射涂层和扫描电子显微镜数字成像。 5.培养细胞提取的、临界点干燥的细胞骨架和附着于底物的膜的铂碳复制品 在过去的一年里，我们为NHLBI/DIR的27名首席研究人员支持了45个项目，为DIR以外的一名科学家支持了6个项目。在此期间，发表了14篇期刊论文，介绍了EM Core的工作，其中12篇EM Core员工是共同作者或EM Core得到认可。我们培训了14名博士后研究员、学生和合同工使用扫描和透射式电子显微镜。

The NHLBI Electron Microscopy Core Facility has supported projects using the following techniques in the past year: 1. Fixation, embedding, ultra-thin sectioning and transmission EM digital imaging of tissues and cells. 2. EM immunocytochemistry, including immunogold localization of proteins and other antigens within and on the surface of cells by pre-embedding and cryo-immunogold techniques. 3. Negative staining of large proteins, polymers and supramolecular structures as well as lipid and membrane vesicles for transmission EM imaging as well as platinum shadowing of large molecules. 4. Fixation, critical point drying, sputter-coating and scanning EM digital imaging of small organisms, organs, tissues and cells, as well as other materials such as artificial matrices. 5. Platinum carbon replicas of extracted, critical point dried cytoskeletons and the substrate-attached membranes of cultured cells In the past year, we have supported 45 projects for 27 principal investigators in the NHLBI/DIR and 6 project for a scientist outside the DIR. During this period, 14 journal articles have been published presenting from work in the EM Core with 12 where the EM Core Staff were co-authors or the EM Core was acknowledged. We have trained 14 postdoctoral fellows, students and contractors in the use of the scanning and transmission electron microscopes.