Animal Physiology Core

动物生理学核心（APC）采用先进的研究方法，以协助爱因斯坦-芒特 西奈糖尿病研究中心（ES-DRC）的研究人员在体内评估葡萄糖和脂肪酸 代谢、胰岛素敏感性和能量稳态。通过与 在ES-DRC的其他核心中，APC使研究人员能够彻底描述定义的 药理学、饮食、环境和遗传改变对葡萄糖和脂质稳态、胰岛素 行动和新陈代谢。核心进行肥胖分布的研究，并促进核磁共振光谱， 进行代谢研究的糖尿病实验动物模型的fMRI和microPET分析。的 核心还提供专门的啮齿动物手术的研究实验室和几个心脏功能 与糖尿病并发症相关的评估。为了实现这些目标，动物生理学核心将：1） 为研究者设计与葡萄糖稳态控制相关的代谢研究提供建议， 胰岛素在啮齿类动物中的作用; 2）向研究者提供全身的专门测量， 组织特异性葡萄糖敏感性和胰岛素作用，包括但不限于胰岛素、胰腺和 高血糖钳夹研究; 3）为研究胰岛素敏感性、能量和血糖提供专门的手术模型。 平衡，葡萄糖和脂肪酸代谢; 4）为学生，博士后研究员， 研究者和技术人员进行必要的手术和生理技术，以评估 葡萄糖稳态和胰岛素作用的控制; 5）提供全身碳水化合物/脂肪酸的分析 氧化，能量消耗，摄食行为和运动活动，使用专门的代谢（间接 量热法）和行为啮齿动物笼; 6）提供自发或计划的影响评估 锻炼对葡萄糖稳态和代谢的影响; 7）向专门的研究人员提供 使用microCT测量啮齿动物脂肪组织分布，并测量肝脏和肝脏中的糖原 肌肉，肝内脂质和肌细胞内脂质使用NMR; 8）提供给研究人员专门 通过功能性MRI（fMRI）和microPET扫描测量脑能量和葡萄糖利用率;以及9） 协调这些努力与其他ES-DRC和机构核心设施在爱因斯坦和西奈山。所有 这些服务提供给新的糖尿病研究人员，以及研究糖尿病的研究人员。 糖尿病相关的项目，可以丰富和扩展的专业知识和设施的使用，这 核心

The Animal Physiology Core (APC) employs sophisticated research methodologies to assist Einstein-Mount Sinai Diabetes Research Center (ES-DRC) investigators in the in vivo assessment of glucose and fatty acid metabolism, insulin sensitivity and energy homeostasis in mice and rats. Through collaborative efforts with the other Cores of the ES-DRC, the APC enables investigators to thoroughly characterize the effects of defined pharmacological, dietary, environmental and genetic alterations on glucose and lipid homeostasis, insulin action, and metabolism. The Core performs studies of adiposity distribution and facilitates NMR spectroscopy, fMRI and microPET analysis of experimental animal models of diabetes undergoing metabolic studies. The Core also provides specialized rodent surgeries for investigator laboratories and several cardiac functional assessments related to diabetic complications. To accomplish these goals, the Animal Physiology Core will: 1) advise investigators in the design of metabolic studies relevant to the control of glucose homeostasis and insulin action in rodents; 2) make available to investigators specialized measurements of whole body and tissue-specific glucose sensitivity and insulin action including, but not limited to insulin, pancreatic and hyperglycemic clamp studies; 3) provide specialized surgical models for the study of insulin sensitivity, energy balance, and glucose and fatty acid metabolism; 4) offer instruction to students, postdoctoral fellows, investigators and technical staff in performing surgical and physiological techniques necessary to evaluate the controls of glucose homeostasis and insulin action; 5) provide analysis of whole body carbohydrate/fatty acid oxidation, energy expenditure, feeding behavior, and locomotor activity using specialized metabolic (indirect calorimetry) and behavioral rodent cages; 6) provide assessment of the effects of spontaneous or scheduled exercise on glucose homeostasis and metabolism; 7) make available to investigators specialized measurements of rodent adipose tissue distribution using microCT and measurements of glycogen in liver and muscle, intrahepatic lipids and intramyocellular lipids using NMR; 8) make available to investigators specialized measurements of brain energy and glucose utilization by functional MRI (fMRI) and microPET scanning; and 9) coordinate these efforts with other ES-DRC and Institutional Core facilities at Einstein and Mount Sinai. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this Core.