Animal Phenotyping (Core B)

动物表型分析（核心 B）

• 批准号: 9259937
• 负责人: GARY J SCHWARTZ
• 金额: $ 23.55万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9259937
• 关键词: Adipocytes; Adipose tissue; Affect; Anatomy; Animals; Autonomic Denervation; Biology; Body Composition; Body Temperature; Body Weight; Brown Fat; Bypass; Calorimetry; Carbon Dioxide; Cell Separation; Characteristics; Complement; Consumption; Data; Development; Diabetes Mellitus; Energy Intake; Energy Metabolism; Equipment; Fatty acid glycerol esters; Feeding behaviors; Functional disorder; Funding; Generations; Genetic; Grant; Histologic; Human; Immunologics; Individual; Laboratories; Liver; Magnetic Resonance Imaging; Maintenance; Manuscripts; Measurement; Measures; Medicine; Metabolic; Metabolic Diseases; Metabolism; Microscope; Mission; Molecular; Molecular Biology; Molecular Genetics; Morphology; Mus; Neuraxis; New York; Nutritional Study; Obesity; Operative Surgical Procedures; Oxygen; Phenotype; Physical activity; Physiology; Procedures; Process; Publications; Rattus; Regulation; Research; Research Personnel; Resources; Rodent; Rodent Model; Running; Scientist; Services; System; Technical Expertise; Temperature; Thermogenesis; Thinness; Tissue Transplantation; Tissues; United States National Institutes of Health; Universities; Variant; Viral; Weight; Weight Gain; Work; adipocyte differentiation; animal resource; base; college; cost; cost effective; design; energy balance; equipment acquisition; fluorescence microscope; gastrointestinal; glucose metabolism; insight; lipid metabolism; macrophage; member; metabolic phenotype; microCT; mouse model; nutrition; operation; optogenetics; prevent; protein metabolism; respiratory; stem; tool

Animal Phenotyping Core PROJECT SUMMARY/ABSTRACT Rodent models of obesity have provide invaluable to the study of obesity and to the work carried out by NYONRC investigators. To meet the growing needs of Center researchers, the proposed NYONRC Animal Phenotyping Core (APC) reflects a significant expansion of the Animal Energy Balance Core (Einstein) and integration with the animal component of the Adipose Tissue Core (Columbia). During the past 4 years of the current cycle, these components of the NYONRC have processed more than 14,000 service requests for 38 NYONRC members supported by 45 grants, and provided additional services to 9 NIH-funded non-NYONRC members. The services offered by the APC have been instrumental in obtaining 19 new or renewed grants, and in the generation of 213 publications. The proposed APC concentrates and coordinates significantly expanded technical and research capabilities of this amalgamated Core. The incorporation of additional animal resources at both the Einstein and Columbia campuses, will significantly increase the user and research base of the NYONRC overall, and of the APC Core in particular. By coordinated application of NYONRC dedicated facilities, and resources and expertise at both Columbia and Einstein, the proposed APC Core will combine sophisticated qualitative and quantitative measurements of ingestive behavior with concurrent assessments of energy expenditure, physical activity, body temperature, and repeatable noninvasive body composition analysis by MRI and MicroCT. Also available are a range of metabolic surgeries, including gastrointestinal bypass, autonomic denervation procedures, and adipose tissue services including: 1) quantifying adipocyte subpopulations (brown, white, beige), in terms of size, variation, and cellular ultrastructure; 2) primary cell isolation and analysis; 3) differentiation of adipocytes and adipose tissue macrophages; and 4) adipose tissue transplantation. This expanded array of services stems from the growing realization that our understanding of the biology of obesity requires simultaneous assessment of energy intake and energy expenditure, and of the means to determine their net effects on energy balance repeatedly in individual animals. The Specific Aims of the APC are to provide state-of-the-art assessments of: 1) Adipose Tissue Morphology & Function, in terms of cellular, histologic and functional analyses; 2) Body Composition, in terms of the total relative fat and lean mass, and whole body anatomical distribution of adipose tissue; 3) Energy intake and Expenditure, including oxygen and CO2 consumption, respiratory quotient, and thermogenesis in brown adipose tissue; and 4) consequences of Metabolic Procedures and Surgeries, designed to illuminate the physiology of weight regulation and obesity pathophysiology, including gastrointestinal bypass, autonomic denervations and central nervous system targeting using viral, optogenetic and chemogenetic approaches..

动物表型核心 项目总结/摘要 啮齿类动物的肥胖模型为肥胖的研究和肥胖研究人员的工作提供了宝贵的参考。 NYONRC调查员。为了满足中心研究人员日益增长的需求，拟议的NYONRC动物 表型核心（APC）反映了动物能量平衡核心（Einstein）的显著扩展， 与脂肪组织核心（哥伦比亚）的动物组分整合。在过去的四年里， 在目前的周期中，NYONRC的这些组成部分已经处理了38个月的14，000多个服务请求 NYONRC成员得到45笔赠款的支持，并为9名NIH资助的非NYONRC成员提供额外服务 成员进通会提供的服务有助于获得19项新的或延长的赠款， 出版了213本书。拟议的APC集中和协调， 扩大了合并后的核心的技术和研究能力。加入额外的动物 在爱因斯坦和哥伦比亚校区的资源，将大大增加用户和研究基地 整个NYONRC，特别是APC核心。通过协调应用NYONRC专用 哥伦比亚和爱因斯坦的设施、资源和专业知识，拟议的APC核心将结合联合收割机 对摄食行为进行复杂的定性和定量测量，同时评估 能量消耗、体力活动、体温和可重复的非侵入性身体成分 通过MRI和MicroCT分析。还提供一系列代谢手术，包括胃肠手术。 旁路、自主神经去神经手术和脂肪组织服务，包括：1）定量脂肪细胞 亚群（棕色，白色，米色），在大小，变化和细胞超微结构方面; 2）初级细胞 分离和分析; 3）脂肪细胞和脂肪组织巨噬细胞的分化;和4）脂肪组织 移植这种服务的扩展源于我们越来越认识到， 肥胖的生物学需要同时评估能量摄入和能量消耗，以及 是指在个体动物中重复测定其对能量平衡的净影响。 APC的具体目标是提供最先进的评估：1）脂肪组织 形态和功能，在细胞，组织学和功能分析方面; 2）身体成分， 总相对脂肪和瘦体重以及脂肪组织的全身解剖分布; 3） 能量摄入和消耗，包括氧气和CO2消耗量、呼吸商，以及 棕色脂肪组织中的产热;和4）代谢过程和表面活性剂的后果， 旨在阐明体重调节的生理学和肥胖的病理生理学，包括 胃肠道旁路、自主神经去神经和使用病毒、光遗传学 和化学遗传学方法。