Animal Phenotyping (Core B)

动物表型核心 项目摘要/摘要 肥胖的啮齿动物模型为肥胖的研究和由 NYONRC调查人员。为了满足中心研究人员日益增长的需求，拟议的NYONRC动物 表型核心(APC)反映了动物能量平衡核心(爱因斯坦)和 与脂肪组织核心的动物成分整合(哥伦比亚)。在过去的4年里， 在本周期内，NYONRC的这些部门已经处理了超过14,000项服务请求 NYONRC成员得到45项赠款的支持，并向9个由NIH资助的非NYONRC提供额外服务 会员。APC提供的服务有助于获得19笔新的或续期的赠款， 并在此基础上出版了213种出版物。拟议的APC显著地集中和协调 扩大了这一合并核心的技术和研究能力。加入额外的动物 爱因斯坦和哥伦比亚大学校园的资源将显著增加用户和研究基础 整个NYONRC，特别是APC核心的。通过协调应用NYONRC专用 哥伦比亚大学和爱因斯坦大学的设施、资源和专业知识，拟议中的APC核心将结合 摄食行为的复杂定性和定量测量，同时评估 能量消耗、体力活动、体温和可重复的非侵入性身体成分 用MRI和MicroCT进行分析。还有一系列新陈代谢手术，包括胃肠手术。 搭桥、自主去神经程序和脂肪组织服务包括：1)量化脂肪细胞 亚群(棕色、白色、米色)，大小、变异和细胞超微结构；2)原代细胞 分离分析；3)脂肪细胞和脂肪组织巨噬细胞分化；4)脂肪组织 移植。这种扩展的服务种类源于我们日益认识到，我们对 肥胖的生物学需要同时评估能量摄入和能量消耗，以及 方法是重复确定它们对个体动物能量平衡的净影响。 APC的具体目标是提供最先进的评估：1)脂肪组织 形态和功能，在细胞、组织学和功能分析方面；2)身体成分， 总相对脂肪和瘦体重，以及脂肪组织的全身解剖分布；3) 能量摄入和支出，包括氧气和二氧化碳消耗、呼吸商和 棕色脂肪组织中的产热作用；以及4)新陈代谢过程和手术的后果， 旨在阐明体重调节的生理学和肥胖的病理生理学，包括 胃肠搭桥术、自主神经失神经和中枢神经系统靶向利用病毒、光遗传 和化学发生方法..

Animal Phenotyping Core PROJECT SUMMARY/ABSTRACT Rodent models of obesity have provide invaluable to the study of obesity and to the work carried out by NYONRC investigators. To meet the growing needs of Center researchers, the proposed NYONRC Animal Phenotyping Core (APC) reflects a significant expansion of the Animal Energy Balance Core (Einstein) and integration with the animal component of the Adipose Tissue Core (Columbia). During the past 4 years of the current cycle, these components of the NYONRC have processed more than 14,000 service requests for 38 NYONRC members supported by 45 grants, and provided additional services to 9 NIH-funded non-NYONRC members. The services offered by the APC have been instrumental in obtaining 19 new or renewed grants, and in the generation of 213 publications. The proposed APC concentrates and coordinates significantly expanded technical and research capabilities of this amalgamated Core. The incorporation of additional animal resources at both the Einstein and Columbia campuses, will significantly increase the user and research base of the NYONRC overall, and of the APC Core in particular. By coordinated application of NYONRC dedicated facilities, and resources and expertise at both Columbia and Einstein, the proposed APC Core will combine sophisticated qualitative and quantitative measurements of ingestive behavior with concurrent assessments of energy expenditure, physical activity, body temperature, and repeatable noninvasive body composition analysis by MRI and MicroCT. Also available are a range of metabolic surgeries, including gastrointestinal bypass, autonomic denervation procedures, and adipose tissue services including: 1) quantifying adipocyte subpopulations (brown, white, beige), in terms of size, variation, and cellular ultrastructure; 2) primary cell isolation and analysis; 3) differentiation of adipocytes and adipose tissue macrophages; and 4) adipose tissue transplantation. This expanded array of services stems from the growing realization that our understanding of the biology of obesity requires simultaneous assessment of energy intake and energy expenditure, and of the means to determine their net effects on energy balance repeatedly in individual animals. The Specific Aims of the APC are to provide state-of-the-art assessments of: 1) Adipose Tissue Morphology & Function, in terms of cellular, histologic and functional analyses; 2) Body Composition, in terms of the total relative fat and lean mass, and whole body anatomical distribution of adipose tissue; 3) Energy intake and Expenditure, including oxygen and CO2 consumption, respiratory quotient, and thermogenesis in brown adipose tissue; and 4) consequences of Metabolic Procedures and Surgeries, designed to illuminate the physiology of weight regulation and obesity pathophysiology, including gastrointestinal bypass, autonomic denervations and central nervous system targeting using viral, optogenetic and chemogenetic approaches..