ANIMAL PHENOTYPING CORE

动物表型核心

• 批准号: 10239752
• 负责人: GARY J SCHWARTZ
• 金额: $ 16.67万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-23 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10239752
• 关键词: Address; Adult; Affective; Anatomy; Animal Disease Models; Animal Model; Animals; Behavior; Behavior assessment; Behavioral; Biological Assay; Body Composition; Body Temperature; Brain; Brain imaging; Child; Collaborations; Complex; Core Facility; Data; Development; Diffusion Magnetic Resonance Imaging; Disease; Eating; Elements; Energy Metabolism; Equipment; Etiology; Experimental Designs; Faculty; Fatty acid glycerol esters; Feeding behaviors; Fostering; Genetic; Genetic Diseases; Goals; Histologic; Human; Image; Imaging Techniques; Impairment; Individual; Insulin; Intellectual and Developmental Disabilities Research Centers; Intellectual functioning disability; Investigation; Leadership; Link; Logistics; Magnetic Resonance Imaging; Measurement; Measures; Metabolic; Metabolism; Methodology; Mission; Monitor; Mus; Mutation; Neurons; Pathogenesis; Pattern; Phenotype; Physical activity; Physiological; Positron-Emission Tomography; Postdoctoral Fellow; Quality Control; Rattus; Regulation; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; Rodent; Rodent Model; Sensorimotor functions; Social Behavior; Statistical Data Interpretation; Structure; System; TNFRSF5 gene; Techniques; Technology; Testing; Training; behavioral phenotyping; behavioral study; blood glucose regulation; career development; cellular engineering; cellular imaging; clinical phenotype; cognitive function; cost effectiveness; data mining; design; disease phenotype; exhaustion; experience; experimental study; feeding; high throughput screening; human disease; innovation; interdisciplinary approach; mature animal; metabolic phenotype; motivated behavior; mouse model; neurogenomics; neuroimaging; neurophysiology; prevent; programs; relating to nervous system; satisfaction; student training; translational approach; whole animal imaging

PROJECT SUMMARY/ABSTRACT – ANIMAL PHENOTYPING Phenotyping animal models of disease is a critical element in our Center’s goal to understand, effectively treat, and when possible, prevent intellectual and developmental disabilities in children. Accordingly, the mission of the Animal Phenotyping Core (Core E, AP) is to assist investigators seeking to discover behavioral, physiological, structural and metabolic phenotypes in diverse rodent models of intellectual and developmental disabilities. The AP Core performs studies primarily in mice and rats to identify the functional alterations resulting from genetic, developmental or environmental manipulations that may impair neural and behavioral development. These include changes in developmental milestones, sensorimotor function, cognitive function, affective and social behaviors, feeding and activity patterns, body composition and/or energy expenditure, patterns of brain activity and anatomy as assessed by brain imaging in MRI/DTI (diffusion tensor imaging) and PET scans. The AP Core accomplishes its goals through SubCores focused on (1) behavior, (2) metabolism, and (3) brain imaging. By combining existing capabilities and highly experienced faculty we have established an animal phenotyping facility uniquely suited to plan, perform and evaluate coordinated behavioral, metabolic, and functional neuroimaging assessments in developing and adult rodents. Through close collaborative efforts with the Neural Cell Engineering and Imaging (NCEI) Core and the Human Clinical Phenotyping (HCP) Core, as well as the Neurogenomics (NGEN) Core, the consequences of defined genetic, environmental and/or physiological alterations are thoroughly characterized to determine their impact in the context of measures most relevant and translatable to the human disease phenotype. The AP Core also makes critical contributions to Aim 2 of the RFK IDDRC signature research project focused on links between mutations in KDM5C and IDD. In addition to the wide range of expertise of its leadership and the resources they bring to this effort, the AP Core also emphasizes the importance of integration across measurement and analytical capabilities, i.e., it facilitates a combination of methodological approaches such as pursuit of brain imaging simultaneously with behavioral studies. We also emphasize, when possible, phenotyping techniques that are most comparable to those used in children with IDD. In pursuit of these scientific objectives, the AP Core leadership, in concert with ADM Core oversight, also carefully monitors IDDRC investigator Core access and user satisfaction, cost effectiveness and quality control.

项目摘要/摘要——动物表型分析 对疾病动物模型进行表型分析是我们中心了解、有效治疗、 并在可能的情况下，预防儿童智力和发育障碍。因此，使命 动物表型核心（核心 E、AP）旨在帮助研究人员发现行为、 不同啮齿动物智力和发育模型的生理、结构和代谢表型 残疾。 AP Core 主要在小鼠和大鼠中进行研究，以确定功能改变 由可能损害神经和行为的遗传、发育或环境操纵引起的 发展。这些包括发育里程碑、感觉运动功能、认知功能的变化， 情感和社会行为、喂养和活动模式、身体成分和/或能量消耗， 通过 MRI/DTI（弥散张量成像）中的大脑成像评估大脑活动和解剖结构的模式 PET 扫描。 AP 核心通过专注于 (1) 行为、(2) 新陈代谢、 (3)脑成像。通过结合现有的能力和经验丰富的教师队伍，我们建立了 动物表型分析设施，特别适合计划、执行和评估协调的行为、代谢、 以及发育中和成年啮齿动物的功能神经影像评估。通过密切的协作努力 具有神经细胞工程和成像 (NCEI) 核心和人类临床表型分析 (HCP) 核心， 以及神经基因组学 (NGEN) 核心，定义的遗传、环境和/或 生理变化被彻底表征，以确定其在措施背景下的影响 与人类疾病表型最相关且可转化。 AP 核心也做出了重要贡献 RFK IDDRC 标志性研究项目的目标 2 重点关注 KDM5C 和 KDM5C 突变之间的联系 国际直拨电话。除了其领导层的广泛专业知识和他们为这项工作带来的资源外， AP Core 还强调了测量和分析能力集成的重要性，即 促进方法论方法的结合，例如同时追求脑成像 行为研究。如果可能的话，我们还强调与最有可比性的表型分析技术 用于 IDD 儿童的药物。为了追求这些科学目标，AP 核心领导层齐心协力 在 ADM 核心监督下，还仔细监控 IDDRC 调查员核心访问和用户满意度、成本 有效性和质量控制。