Tissue Core

成瘾治疗神经生物学中心 动物和组织核心总结 博士Mark Ferris，核心总监; Paul Czoty博士，共同研究者 动物和组织核心的总体目标是生成、跟踪和分配组织， 实验动物进行多项体内和体外实验。核心将集中和标准化 啮齿动物脑组织的生成、储存和转移到项目2和3。这些研究包括自我- 施用、病毒介导的多巴胺能D2受体的基因敲低和用多巴胺能D2受体的急性/慢性治疗 项目1中确定的假定药物。通过对这些动物的产生负责， 在项目2和3的研究中，核心将确保所有受试者将在一个 标准化的方式。大脑和周围组织将由核心组织和储存，这将确保 准确记录受试者的行为和药理学史。内核会把这些组织 在盲态条件下，对项目2和3的研究者进行成像和生化研究。小心 这些样品的制备和处理以及信息的精心组织是必需的， 从使用这些组织样本的实验中可以获得可靠的结论，这使得 动物和组织核心是中心的重要组成部分。具体目标是： 1.为了产生具有针对D2受体的病毒介导的基因敲低的大鼠组， 项目2和3。 2.产生具有可卡因自我给药史（有和无药物）的大鼠组 治疗和病毒介导的基因敲减）。 3.为保存在核心内的所有组织提供详细记录的中心位置，并 在双盲条件下及时向项目2和项目3分发该组织。 4.为了收集和储存将由可卡因自我给药研究产生的啮齿动物大脑， 项目1：推定治疗药物的生化分析和其他潜在的未来用途。 5.采集和储存非人灵长类动物血液和脑脊液（CSF），用于代谢物分析 假定的治疗药物和其他潜在的未来用途，以及非人类灵长类动物大脑样本库 项目1的研究完成后， 6.维护组织库并定期活检组织，以确保组织完整性。

Center for the Neurobiology of Addiction Treatment Animal and Tissue Core Summary Dr. Mark Ferris, Core Director; Dr. Paul Czoty, co-investigator The overall objective of the Animal and Tissue Core is to generate, track and distribute tissue from experimental animals to multiple in vivo and in vitro experiments. The Core will centralize and standardize the generation, storage and transfer of brain tissue from rodents to Projects 2 and 3. These studies include self- administration, viral mediated gene knockdown of dopaminergic D2 receptors and acute/chronic treatment with putative medications identified in Project 1. By taking responsibility for the generation of these animals for studies in Projects 2 and 3, the Core will ensure that all subjects will be treated and euthanized in a standardized manner. Brain and peripheral tissue will be organized and stored by the Core, which will ensure accurate recording of the subjects' behavioral and pharmacological histories. The Core will distribute this tissue under blinded conditions to investigators in Projects 2 and 3 for imaging and biochemical studies. Careful preparation and handling of these samples and meticulous organization of information are required to ensure that reliable conclusions can be obtained from experiments using these tissue samples, which makes the Animal and Tissue Core an essential component of the Center. The specific goals are: 1. To generate groups of rats with viral mediated gene knockdown targeted against D2 receptors for Projects 2 and 3. 2. To generate groups of rats with a history of cocaine self-administration (with and without medication treatment and viral mediated gene knockdown) for Projects 2 and 3. 3. To provide a central locus for detailed record-keeping for all tissues stored within the Core and to provide timely distribution of this tissue under double-blinded conditions to Projects 2 and 3. 4. To collect and bank rodent brains that will be generated by cocaine self-administration studies in Project 1 for biochemical analyses of putative treatment drugs and other potential future uses. 5. To collect and bank nonhuman primate blood and cerebrospinal fluid (CSF) for analysis of metabolites of putative treatment drugs and other potential future uses, and bank nonhuman primate brain samples as they become available after studies in Project 1 are completed. 6. To maintain the tissue bank and periodically biopsy tissue to ensure tissue integrity.