CCR-Frederick Flow Cytometry Core

CCR-Flow核心是NCI和弗雷德里克国家癌症研究实验室的承包商研究人员不可或缺的资源。来自30个不同实验室的97名研究人员利用了流动岩心工作人员的专业知识以及工作人员可用和维护的仪器。流式细胞术核心人员和仪器支持以下研究：使用带有tdtomato荧光蛋白的RAG转基因小鼠对共生体适应性免疫的分选支持，MDSC和调节性T细胞在抗cd40肿瘤治疗中的作用，Id2和gfI-1对造血干细胞红细胞生成的调节；证明微生物群在自身免疫性疾病中的作用；显示TNFalpha对CD4+Foxp3+调节性T细胞的抑制活性以及IKKalpha对这些T调节性细胞的稳态作用；ifnγ改变造血干细胞分化在再生障碍性贫血中的作用Lewis肺癌巨噬细胞的串扰及红系祖细胞的转录和Id2抑制的研究。这些仪器每天都由训练有素的调查人员使用，经常使用到深夜和周末。流动核心一直在培训CIP以外的调查人员分析他们自己的样本，扩大用户/操作员的基础。这使得flow core人员在今年最后10个月有更多的时间对大约424个样品进行分类，并分析大约8474个样品。如果没有调查人员获取数据并分析他们自己的样本，政府将不得不聘请有经验的全职人员来完成同样数量的工作。在过去的一年里，至少有13篇论文使用了从流动岩心中获得的数据。未来的目标包括通过继续扩大经过培训的研究人员的数量，以运行和分析弗雷德里克所有CCR的自己的样品，从而扩大使用核心仪器和专业知识产生的科学的使用，生产力和质量，从而跟上流动实验室的尖端技术。我们已经升级了所有的仪器，使激光和荧光功能更容易互换，使研究人员能够灵活地使用多种仪器。这将防止由于所需要的仪器正在被另一名调查人员使用或因需要维修而停机而丢失数据。细胞分选器也进行了升级，以允许直接翻译在分析仪上开发的实验到细胞分选器，以分选出待进一步研究的感兴趣的细胞群。在样品分析和细胞分选中具有这种灵活性是至关重要的。我们还想保留一个尖端的设施，这将需要至少一个分析仪升级，一次分析至少28个参数。

The CCR-Flow Core is an indispensable resource for NCI and contractor investigators at the Fredereick National Lab for Cancer research. ninety seven investigators from 30 different laboratories have used the expertise of the flow core staff and the instrumentation available and maintained by the staff. The Flow Cytometry Core staff and instrumentation have supported the following studies: sorting support for adaptive immunity of commensals using RAG transgenic mice with a tdtomato fluorescent protein, the role of MDSC's and regulatory T cells in anti-CD40 tumor therapy, the regulation of erythropoiesis of hematopoietec stem cells by Id2 and gfI-1; demonstrating the role of microbiota in autoimmune disease; showing the suppressive activity of CD4+Foxp3+ regulatory T cells by TNFalpha and the homeostatic effects of IKKalpha on these T regulatory cells; role of IFNgamma altering hematopoietic stem cell differentiation in aplastic anemia; studies on the crosstalk of macrophages in Lewis lung carcinoma and the erythroid transcription and Id2 repression of erythroid progenitors. The instruments are used daily by the trained investigators often into the night and on the weekends. The flow core has been training investigators outside of the CIP to analyze their own samples expanding the base of user/operators. This leaves more time for the flow core staff to sort approximately 424 samples and analyze about 8474 samples in the last 10 months of this year. Without the investigators acquiring the data and analyzing their own samples, the government would have to hire full time experienced individuals to perform the same volume of work. At least 13 papers have been published in the past year using data obtained in the flow core. The future goals include keeping up with cutting-edge technology in the flow lab by continuing to expand the pool of investigators trained to run and analyze their own samples to all of CCR in Frederick thereby expanding the use, productivity and quality of the science generated using the core's instrumentation and expertise. We have up-graded all of the instruments so that laser and fluorochrome capabilities are more interchangeable giving investigators the flexability to use more than one instrument. This would prevent loss of data because the instrument needed was in use by another investigator or down because of needed repairs. The cell sorters have also been up-graded to allow direct translation of an experiment developed on an analyzer to the cell sorter to sort out the populations of cells of interest to be further studied. It is critical to have this flexability in analysis of samples as well as for sorting of cells. We would also like to stay a cutting edge facility which would require at least one analyzer to be up-graded to analyze at least 28 parameters at once.