Shared Resource: Genomic and RNA Profiling

基因组和RNA分析共享资源（组学集团） 项目摘要 DLDCC的基因组和RNA分析共享资源（GARP SR）致力于满足学术 癌症中心研究人员的需求，并保持灵活性，以帮助开发新的应用程序。这是一 这是一个充满活力的设施，与新兴技术和癌症中心不断变化的需求保持同步。 DLDCC研究人员已经能够通过利用国家， 最先进的技术，包括基于微阵列和深度测序的基因组和转录组分析 工具.这项研究涉及异常表达，基因组广泛表达干扰，或 转录后基因调控这些工具的范围从全基因组检测到单个基因检测。 分析和整个组织到单细胞。一个例子是中等吞吐量多路复用的实现 技术，如NanoString nCounter数字定量系统，可以分析多达800多个 在很短的标准化时间内完成分子靶点。该平台帮助癌症中心的研究人员， 远离全基因组筛选或从Illumina测序中识别基因签名， 现在集中在可能预测癌症进展的基因或途径的特征集上。这些 然后，签名可以用来开发和测试新的药物，旨在干扰特定的基因， 产品或信号通路。GARP SR致力于寻找合适的工具来帮助癌症中心的研究人员， 癌症是一种复杂的疾病。我们已经帮助许多研究人员从基本的过渡 研究转化研究，努力通过提供获得 新技术有大量的DLDCC研究人员需要提供的方法 这些资源来进行研究。基因组谱分析实验的成本可能是令人望而却步的， 个人调查员无法获得共享资源。数据分析需要统计学经验， 生物信息学和这一资源汇集了为DLDCC提供坚实基础的个人团体 研究人员，这将使他们能够成功地利用基因组技术，这将不会是 如果没有这个共享资源。

CORE: Genomic and RNA Profiling Shared Resource (Omics Group) PROJECT SUMMARY The Genomic and RNA Profiling Shared Resource (GARP SR) of the DLDCC works to meet the academic needs of cancer center investigators and remain flexible to aid in developing new applications. This is a dynamic facility that has kept pace with emerging technologies and the changing needs of the cancer center. DLDCC investigators have been able to make significant advancements in cancer research by utilizing state- of-the-art technologies including microarray- and deep sequencing-based genomic and transcriptomic analysis tools. This research has involved studies of aberrant expression, genome wide-perturbations of expression, or post transcriptional gene regulation. These tools range from whole genome wide assays to individual gene assays and whole tissue to single cell. An example is the implementation of medium throughput multiplexing technologies such as the NanoString nCounter digital quantitation system that can assay up to 800 multiplexed molecular targets in a very short, standardized time. This platform helps cancer center investigators who are moving away from genome-wide screening or who identify a gene signature from Illumina sequencing and are now focusing on the signature sets of genes or pathways that may predict cancer progression. These signatures could then be used to develop and test novel drugs designed to interfere with specific gene products or signaling pathways. GARP SR strives to find the right tools to help cancer center investigators to understand cancer as a complex disease. We have aided many researchers in a transition from basic research into translational research in an effort to bring their discoveries to the clinic by providing access to new technologies. There are a large number of DLDCC researchers who require the methodologies offered in this resource to carry out their studies. Costs for genomic profiling experimentation can be prohibitive to the individual investigator without access to a shared resource. Data analysis requires experience in statistics and bioinformatics and this resource brings together groups of individuals who provide a solid base for DLDCC researchers that will enable them to successfully utilize genomic technology—something that would not be possible without this shared resource.