Host-Pneumococcal Interaction in the Lung

描述（由申请人提供）：细菌性肺炎和败血症是重症监护病房中可预防的主要死亡原因，肺炎链球菌（Sp）是主要病因。目前的治疗方法主要集中在抗生素的使用上，这导致了耐药性和高毒力菌株的迅速出现。成功的病原体-宿主相互作用的一个基本方面是在成功清除病原体的同时保持有害的、组织损伤的炎症免疫反应的能力。细菌感染引起的高发病率和死亡率可能是炎症失调的结果。目前还没有可行的治疗方式来增强宿主防御，同时减少不必要的炎症。此外，人们对信号级联如何收敛以控制宿主防御同时最小化炎症组织损伤知之甚少。考虑到这一点，我们已经确定了几丁质酶3-样1 (Chi3l1)，这是18-糖基水解酶家族的一种原型几丁质酶样蛋白，在这一过程中起重要作用。我们发现Chi3l1

DESCRIPTION (provided by applicant): Bacterial pneumonia and sepsis are leading preventable causes of death in the intensive care unit and Streptococcus pneumoniae (Sp) is a major etiology. Current therapies are mostly focused on the use of antibiotics and have led to the rapid emergence of drug resistance and hypervirulent strains. A fundamental aspect of successful pathogen-host interactions is the ability to keep harmful, tissue-damaging inflammatory immune responses in check while successfully clearing the pathogen. The exaggerated morbidity and mortality seen with bacterial infection can be the result of dysregulated inflammation. There are currently no viable therapeutic modalities to augment host defense while reducing unnecessary inflammation. Further, it is poorly understood how signaling cascades converge to control host defenses while minimizing inflammatory tissue injury. With this in mind, we have identified Chitinase 3-like 1 (Chi3l1), a prototypic Chitinase-like protein of 18- glycosyl hydrolase family, to be important in this process. We find that Chi3l1 is a potent innate immune regulator elevated in patients with diseases characterized by inflammation and tissue repair. Chi3l1, along with its newly discovered receptor IL13RD2, is induced during Sp infection and is critical in antibacterial responses. The Chi3l1-IL13RD2 axis is important as a therapeutic target for controlling potentially harmful innate immune activation during bacterial infection. Our data show that the Chi3l1-IL13RD2 interaction critically dampens potentially harmful type I interferon (IFN-1) responses during infection. The absence of Chi3l1 or IL13RD2 results in heightened IFN-1s during infection associated with increased co-localization of endosomal Toll-like receptor 9 (TLR9) and interferon regulatory factor 7 (IRF7). Our overall hypothesis is that the Chi3l1-IL13RD2 dampens excessive inflammation and tissue injury by regulating IFN-1 pathway. We will test this hypothesis with the following specific aims: Specific Aim 1. Characterize the mechanisms by which Chi3l1 and its receptor IL13RD2 regulate IFN-1 production and signaling in a murine model of bacterial infection. Specific Aim 2. Examine the role of Chi3l1 and IL13RD2 in the regulation of endosomal trafficking of TLR9 during bacterial infection in vitro and in a mouse model. Specific Aim 3. Determine which cells are critically required for Chi3l1 and IFN-1 production during infection, and determine the clinical significance of Chi3l1 and IFN-1 in bacterial infection. This project aims to understand the mechanisms of pathogen-related immunopathology during bacterial infection, including the mechanisms by which Chi3l1 modulates the IFN-1 response to control immunopathology. We will also explore the role of Chi3l1 in endosomal trafficking of TLRs during infection and assess the use of Chi3l1 as a prognostic biomarker in infected patients. The data generated from this application are expected to provide novel insights that promote the development of new therapies for bacterial infection.