Basic-Optical Biology Core (OBC)

CORE 004-基础-项目摘要/摘要-光学生物学核心(OBC) 光学生物学核心由一个自用设施(在麦高霍尔)和一个基于协作的方法组成 位于贝克曼激光研究所和医疗诊所(BLIMC)的开发设施，即激光 微束和医疗程序设施(LAMMP)，一个NIBIB生物医学技术资源中心。这个 自用设施包括蔡司LSM 780和LSM 700，可以对活的或固定的材料进行成像。双光子 用于深部组织成像的激光和用于成像从DAPI(405 Nm)到远距离的所有荧光团的单光子激光 780上提供红色(633 Nm)。一种用于研究的薄膜荧光寿命显微镜探测器 基于其荧光寿命的分子提供了一种研究任何情况的方法 使用FRATH。赛义德博士管理这个设施，并提供培训和现场故障排除以及 实验性的建议。还提供了对Volocity图像分析软件包的培训，即 可在高端工作站或个人办公室计算机上使用。网页链接为http://obc.bio.uci.edu. OBC的第二个分支包括LAMP设施。NIBIB生物医学技术资源中心 可用于使用多种显微镜和技术进行协作使用和协议开发 由该中心开发。有关这些功能的信息，请访问http://lammp.bli.uci.edu/. 这些技术包括用于研究皮肤癌的多维显微镜(MDM)平台、 非侵入性检测早期黑色素瘤，使用可见光(漫反射光学)进行乳腺癌筛查 光谱学和成像(DOSI)而不是X射线。Krasieva博士管理着这个设施，可以 为寻求新的成像应用的研究人员提供咨询和方案开发。他们受到支持 通过允许建造和改装成像平台的商店设施。

Core 004 - Basic - Project Summary/Abstract - Optical Biology Core (OBC) The Optical Biology Core consists of a self-use facility (in McGaugh Hall) and a collaboration-based methods development facility located in the Beckman Laser Institute and Medical Clinic (BLIMC) namely, the Laser Microbeam and Medical Program facility (LAMMP), a NIBIB Biomedical Technology Resource Center. The self-use facility contains a Zeiss LSM 780 and a LSM 700 that can image living or fixed material. A two photon laser for deep tissue imaging and single photon lasers for imaging all fluorophores from DAPI (405nm) to far red (633nm) are available on the 780. A FLIM (Fluorescence Lifetime Microscopy) detector for studying molecules based on their fluorescence lifetime provides a way of studying any situation that would typically employ FRET. Dr. Syed manages this facility and provides training and on-site trouble shooting as well as experimental advice. Training is also provided for the Volocity image analysis software package that is available on a high end work station or from individual office computers. The web link is http://obc.bio.uci.edu. The second arm of the OBC includes the LAMMP facility. This NIBIB Biomedical Technology Resource Center is available for collaborative use and protocol development using a number of microscopes and technologies being developed by that center. Information about the capabilities can be found at http://lammp.bli.uci.edu/. These include Multi Dimensional Microscopy (MDM) platforms for studying skin carcinomas, techniques to detect early melanoma non-invasively, and breast cancer screening using visible light (diffuse optical spectroscopy and imaging, DOSI) rather than X-rays. Dr. Krasieva manages this facility and is available for consultation and protocol development for investigators seeking new imaging applications. They are supported by shop facilities that allow construction and modification of imaging platforms.