Biosafety Level 3 Core

结核病和艾滋病毒感染的交叉正在增加这两种疾病的全球负担。HIV感染 大大增加了结核病从潜伏性感染转化为活动性感染的风险， 艾滋病毒阳性人群的发病率和死亡率。2006年，难民署主办了一次关于TBHIV的全国会议， 共同感染，这导致了HU CFAR成员工作组的成立，重点关注TB-HIV 研究，并确定一个主要的未满足的需求：BSL-3生物遏制设施，将允许细胞 分类和成像。当Ragon研究所的新设施正在建设时，HU CFAR 成员成功地请求菲利普T和苏珊M拉根基金会，包括一个国家的最先进的 BSL-3实验室满足这些需求。这些最近完成的设施包括2240平方英尺的BSL- 3层空间，流式细胞术和细胞分选，动物处理，成像和额外的空间， 提供临时设备。在HU CFAR的支持下，该设施现在是新创建的HU的所在地 CFAR BSL-3核心。丹尼尔卡瓦纳博士担任PI，与Sarah Fortune博士密切合作， 国际公认的结核病研究专家，担任新成立的Ragon TB主任 计划，与HU CFAR TB-HIV科学工作组密切互动。福琼博士和 Kavanagh共同监督一个由研究员、科学家和一名设施经理组成的团队， 具有BSL-3级安全生产研究经验。这支球队的综合才能，以及 国家的最先进的BSL-3设施，是，提供给胡CFAR社区。为HU CFAR用户提供 培训和认证，以便在设施内进行实验;或者，核心工作人员可以 代表不希望进行组织培养和微生物学研究的研究人员进行标准的组织培养和微生物学程序， 在设施内工作。拟议的HU CFAR生物安全3级核心由以下具体指导 目的： 1.为正在计划的HU CFAR社区成员提供培训和咨询 结核病相关项目 2.提供BSL-3微生物学、组织培养、活细胞显微镜、活流式细胞仪、动物 设施和特殊项目。

The intersection of TB and HIV infection is increasing the global burden of both diseases. HIV infection greatly increases the risk of TB converting from latent to active infection, and TB infection increases morbidity and mortality in the HIV+ population. In 2006, the HU CFAR sponsored a national meeting on TBHIV coinfection, which led to the creation of a working group of HU CFAR members focused onTB-HlV research, and identification of a major unmet need: a BSL-3 biocontainment facility that would allow for cell sorting and imaging. When new facilities for the Ragon Institute were being constructed, the HU CFAR Membership successfully petitioned the Phillip T and Susan M Ragon Foundation to include a state of the art BSL-3 laboratory to meet these needs. These recently completed facilities include 2240 square feet of BSL- 3 level space, flow cytometry and cell sorting, animal handling, imaging and additional space to accommodate temporary equipment. With HU CFAR support this facility is now home of a newly created HU CFAR BSL-3 Core. Dr. Daniel Kavanagh serves as the PI, working closely with Dr Sarah Fortune, an internationally recognized expert in studies of TB who serves as Director of the newly-created Ragon TB Program, with close interactions with the HU CFAR TB-HIV Scientific Working Group. Drs. Fortune and Kavanagh jointly supervise a team of fellows, staff scientists, and a facility manager who bring extensive experience in safe and productive of BSL-3-level research. The combined talents of this team, as well as state-of-the-art BSL-3 facilities, are, available to the HU CFAR community. HU CFAR users are offered training and certification to conduct experiments within the facility; alternatively Core staff are available to carry out standard tissue culture and microbiology procedures on behalf of researchers who do not wish to work inside the facility. The proposed HU CFAR Biosafety Level 3 Core is guided by the following specific aims: 1. Provide training and consultation for members of HU CFAR community members who are planning TB related projects 2. Provide access to BSL-3 microbiology, tissue culture, live cell microscopy, live FACS, animal facilities, and special projects.