Age-related Structural Analyses Core

项目摘要 国家老龄研究所的任务包括阐明原因以及治疗 衰老的方方面面。年龄相关性听力损失(ARHL)是与以下疾病相关的最常见疾病之一 衰老。事实上，患有ARHL的人数超过了任何其他获得性听力损失。这个 这种衰老病理的流行是一个令人信服的指标，表明需要确定糖尿病的深入机制 ARHL，并提供可缓解沟通障碍的综合战略。这个 流行的说法表明ARHL的病因是衰老的神经机制所固有的。神经元 退行性变作为ARHL的一个持久特征，在档案人类颞骨分析中比比皆是，提供了 以螺旋神经节神经元(SGN)为靶点的令人信服的理由。本申请的总体目标 目的是研究感觉神经性急性淋巴细胞性白血病的早期启动机制，并提出治疗方法。 听觉功能的恢复。 核心C的目的是支持项目1至3促进知识的发展，在蜂窝和 第8神经的分子神经学水平，其突触，Corti器的输入细胞和 罗森塔尔管。Core C的任务是评估毛细胞与年龄相关的超微结构变化-SGN SG细胞中的突触，以及Corti器官中紧密连接的完整性。第八神经的研究， 在主要项目中进行的SGC和HC功能通过高分辨率和/或超微结构得到增强 生物标本的特征。为此，项目调查员可以获得综合 核心C的服务包括全面的电子显微镜和成像技术。这些 服务还包括组织准备和图像采集服务，用于结构/超微结构分析 因为听觉外周蛋白质的超微结构定位，从而使项目研究人员能够 专注于对同一组织进行创新的细胞和分子分析。 核心服务允许集中提供显微镜和数字成像服务。这个 复杂组织制备和随后的高质量电子显微镜染色的可用性 技术人员与精通冷冻骨折和电子断层扫描的科学家合作确保 使用经典和尖端技术进行一致的数据收集。核心主管保证，大多数 采用了适当的实验设计和数据分析。适当审查以下请求 结构分析确保在用户之间公平和公平地分配资源。

Project Summary The mission of the National Institute on Aging includes elucidation of the causes as well as treatment of all aspects of aging. Age-related hearing loss (ARHL) is one of the most common ailments associated with aging. In fact, the number of people with ARHL exceeds that of any other acquired hearing loss. The prevalence of this aging pathology is a compelling indicator of the need to identify the in-depth mechanisms of ARHL and to provide comprehensive strateties with which the communication disability can be alleviated. The prevailing account suggests that the etiology of ARHL is intrinsic to the neural mechanisms of aging. Neuronal degeneration as a persistent feature of ARHL abounds in archival human temporal bone analyses, providing a compelling rationale for targeting spiral ganglion neurons (SGN). The overall aims of the present application are to investigate the very early, initiating mechanisms of sensorineural ARHL and to propose avenues for restoration of auditory function. The purpose of Core C is to support Projects 1 through 3 in advancing knowledge, at the cellular and molecular neuroscience level in the 8th nerve, its synapses, cells of input in the organ of Corti and cell bodies in Rosenthal’s canal. Core C's mission is to assess age-related ultrastructural changes at the hair cell-SGN synapses, in the SG cell as well as the integrity of the tight junctions in the organ of Corti. Studies of 8th nerve, SGC and HC function conducted in the main Projects are enhanced by high resolution and/or ultrastructural characterizations of the biological specimen. To this end, the Project investigators have access to integrated services of Core C that include a comprehensive range of electron microscopy and imaging techniques. These services include tissue preparation and image acquisition services for structural/ultrastructural analysis as well as ultrastructural localization of proteins in the auditory periphery thus allowing the Project investigators to focus on innovative cellular and molecular analyses of the same tissue. The Core services allow a centralized source for microscopy and digital imaging services. The availability of complex tissue preparation and subsequent staining by a highly qualified electron microscope technician, collaboration with scientists well-versed in freeze-fracture and electron tomography assures consistent data collection using classical and cutting edge technology. The Core Director assures that the most appropriate experimental designs and data analysis have been utilized. Appropriate review of requests for structural analyses assures that there is a fair and equitable distribution of resources among users.