Mouse Genetic Models for Alcohol Excessive Drinking and Dependence

 描述(由申请者提供)：我们为酒精研究保留了大量独特的小鼠基因类型。这些动物被用于由多个R01、U01和几个VA功绩审查补助金支持的研究。这些动物已经被波特兰、俄勒冈州和世界各地的许多其他地点的研究人员用于研究。对这些基因型的部分支持来自波特兰酒精研究中心(PARC)P60拨款，克拉布博士的VA功绩审查拨款，并已在雇用他们的主要拨款中列入预算。费用膨胀以及赠款周期和实际使用之间缺乏同步，导致我们在支持这些老鼠的能力方面越来越落后。我们无法跟上的部分原因是通货膨胀超过了NIH可乐的限制，部分原因是几个主要支持拨款(P60、U01和VA功绩审查)的预算上限。此外，PARC P60赠款在2016年1月1日续签时将不再使用这些基因类型，因此这种成本分担来源将失去。拟议的R24寻求适度的支持，以保持多种基因类型，在每种情况下，费用与使用费和来自父母赠款的维护支持分摊。目标1.帮助维持长期选择的品系及其遗传异质性控制系(WSP-1、WSP-2、WSR-1、WSR-2、WSC、HDID-1、HDID-2和HS/NPT)的核心育种群体。目的2.为数量性状基因座(QTL)的基因定位研究提供帮助，帮助维持同源品系和品系以及转基因或基因敲除品系的核心育种群体。目的3.部分支付长期选择品系和同源及转基因品系的超低温保存费用。超低温保存可防止关键基因型的灾难性丧失，并为未来的潜在用途提供通道[选定品系WSP-1、WSP-2、WSR-1、WSR-2、HDID-1、HDID-2和敲除B6.GIRK3-/-]。目的4.部分支付维持乙醇蒸气吸入核心以产生身体依赖的费用。这一核心支持多项赠款和调查人员。PARC P60赠款的费用分摊将不再可用，因为更新中的PARC研究将不会使用身体依赖。所有涉及的基因类型都提供给感兴趣的研究人员。没有申请支持分配的资金，因为这种支持是在支持各种基因类型的主要赠款项下提供的。支持繁殖和测试额外动物的资金也不是这里要求的，而是根据父母的赠款。

 DESCRIPTION (provided by applicant): We maintain a large number of unique genotypes of mice for alcohol research studies. These animals are used in research supported by multiple R01s, U01s, and several VA Merit Review Grants. These animals have been used for research by investigators in Portland, Oregon and at many other sites around the world. Partial support for these genotypes has come from the Portland Alcohol Research Center (PARC) P60 grant, Dr. Crabbe's VA Merit Review grant, and has been budgeted within the primary grants employing them. Cost inflation and the lack of synchrony between grant cycles and actual use has led us to fall further and further behind in our ability to support these mice. Our inability t keep up is due partly to inflation exceeding the NIH COLA limits, and partly to budget caps on several of the principal supporting grants (P60, U01s, and VA Merit Reviews). Furthermore, the PARC P60 grant will no longer be using these genotypes when it renews Jan 1 2016, so this source of cost-sharing will be lost. The proposed R24 seeks modest support to maintain multiple genotypes, in each case cost-shared with user fees and maintenance support from the parent grants. There are four aims: Aim 1. Help maintain core breeding colonies of long-term selected lines and their genetically heterogeneous control lines (WSP-1, WSP-2, WSR-1, WSR-2, WSC, HDID-1, HDID-2, and HS/Npt). Aim 2. Help maintain core breeding colonies of congenic strains and lines and transgenic or knockout strains, for quantitative trait locus (QTL) gene mapping studies. Aim 3. Partially defray costs for cryopreservation of long term selected lines and congenic and transgenic strains. Cryopreservation protects against catastrophic loss of crucial genotypes as well as providing access for potential future use [selected lines WSP-1, WSP-2, WSR-1, WSR-2, HDID-1, HDID-2, and the knockout B6.GIRK3-/-]. Aim 4. Partially defray costs of maintaining an ethanol vapor inhalation core for producing physical dependence. This core supports multiple grants and investigators. Cost-sharing from the PARC P60 grant will no longer be available as PARC studies in the renewal will not employ physical dependence. All genotypes covered are made available to interested investigators. Funds to support distribution are not requested, as this support is provided under the primary grants supporting the various genotypes. Funds to support breeding and testing of additional animals are also not requested here, but rather under the parent grants.