ShEEP Proposal for a Multiuser Advanced Biosafe Flow Cytometer

摘要 本申请针对的是BD FACSAria Fusion流式细胞仪，该流式细胞仪将作为多用户 在山居医疗中心的核心设施的仪器。该系统将大大 加强对正在进行VA资助研究的主要用户的研究， 小用户。虽然该细胞仪将主要用于VA资助的研究，但仪器时间 还将提供给新的调查人员和寻求获得初步数据的调查人员 提交VA补助金。山居退伍军人医疗中心目前没有可靠的 多用户流式细胞仪，目前使用的是老化的FacsCalibur流式细胞仪， 于2000年购买，由奎伦医学院拥有和经营; 细胞计数器继续被参与这项研究的研究人员广泛使用， 应用程序，它有有限的颜色选择，没有分拣能力，必须收费。所有 退伍军人管理局和大学研究人员依赖于这种流式细胞仪。细胞计数技术 在过去的几年里取得了巨大的进步， 目前要求我们采用最先进的细胞计数技术， 在我们现有的仪器上。我们现在的细胞仪 不能满足退伍军人管理局资助的调查人员的需求，其中许多人不仅需要分类， 功能，而且是一个集成的生物安全柜，以满足生物保护的要求 在翻译研究中，此外，没有流式细胞仪可与 BD FACSAria融合，在此应用程序中要求，在我们VA的200英里半径内。 因此，该应用解决了重大的研究需求。山居退伍军人事务部 对流式细胞仪的购置和维护做出强有力的机构承诺。一 500平方英尺已经分配了适当配备以容纳仪器的房间。 已作出规定，一旦初始保修期结束， 已过期.成立了一个咨询委员会。博士水平的科学家， 具有流式细胞术经验，将在经验丰富的 研究技术员这一工具将大大改善山区的研究工作 家庭VA旨在改善退伍军人的健康。

Abstract This request is for a BD FACSAria Fusion flow cytometer which will function as a multiuser instrument in a core facility at the Mountain Home Medical Center. The system will significantly enhance the research of major users who are conducting VA funded research and multiple minor users. While this cytometer will primarily be used for VA funded research, instrument time will also be provided to new investigators and investigators seeking to obtain preliminary data for submission of VA grants. The Mountain Home VA Medical Center currently has no reliable multiuser flow cytometer, and currently utilizing an aged FacsCalibur flow cytometer that was purchased in 2000 and which is owned and operated at the Quillen College of Medicine; while the cytometer continues to be used extensively by the investigators participating in this application, it has limited color options, no sorting capacity, and must be fee-based. All of the VA and University investigators are dependent on this flow cytometer. Cytometric technology has advanced dramatically in the last several years and the research questions that we are currently asking require that we employ state-of-the-art cytometric techniques that are not available on our current instrument. We are clearly at the point where our current cytometer does not meet the needs of VA funded investigators, many of whom require not only sorting capabilities but also an integrated biosafety cabinet to meet the requirements of bioprotection inherent in their translational studies. Furthermore, there are no flow cytometers comparable to the BD FACSAria Fusion, requested in this application, within a 200 mile radius of our VA. Therefore, this application addresses a significant research need. The Mountain Home VA has a strong institutional commitment to the acquisition and maintenance of the flow cytometer. A 500 sq. ft. room, that is appropriately outfitted to house the instrument, has been allocated. Provisions have been made to underwrite the service contract once the initial warranty period has expired. An advisory committee has been established. A doctoral level scientist, with experience in flow cytometry, will supervise the facility with the assistance of an experienced research technician. This instrumental will significantly improve the research efforts at Mountain Home VA aimed at improving veterans' health.