Aging and Transgenic Animal Core (Core C)

衰老和转基因动物核心（核心C）将继续支持体内实验的需要， 本项目的四个项目的小鼠模型。 该核心的长期目标是为项目提供广泛的年龄控制动物 在不同组织中具有修饰的自噬功能的模型，并开发用于研究的新小鼠模型 自噬的机制。这些动物已经并将继续成为表演的必需品 所有四个项目中包含的研究，将描述与年龄有关的变化的后果， 自噬作用在细胞生物学和器官及系统功能的不同方面。 核心的具体目标是：1）产生和维持具有改变的转基因动物， 自噬在不同组织中; 2）维持野生型和遗传修饰的年龄控制的集落 3）通过协调牺牲，在计划项目的研究人员之间发挥联系作用。 通过整合从不同动物模型获得的信息， 4）促进所有项目中纳入的小鼠干预措施的统一性 在这个新的时期; 5）收集一些共享的转基因小鼠模型的寿命信息 通过所有项目，并整合来自相同动物的每个项目的健康跨度信息; 6）工作 在新型CMA报告小鼠模型的表征中与核心B密切相关。 该中心提供的服务包括：小鼠群体的维护，基因分型的协调， 动物治疗（饮食和药物化合物）的给药和监测，动物 解剖、收集组织样本用于储存或组织病理学分析，以及连续获取 可搜索的动物数据库 核心的关键人员是主任，由动物技术员协助。主任 监督核心的所有活动，批准PP成员使用动物，建立育种 计划，以适应项目的需要，并监督动物信息输入数据库。的 技术人员执行与动物群体维护，基因分型， 治疗、组织收集、行为测试和将信息输入数据库。

The Aging and Transgenic Animal Core (Core C) will continue to support the need for in vivo experimental mouse models of the four projects of this Program Project. The long-term goals of this Core is to provide the projects with a wide repertoire of age-controlled animal models with modified autophagic function in different tissues and to develop new mouse models for the study of autophagy in a whole organisms. These animals have been and will keep being essential for performing studies contained in all four projects that will characterize the consequences of the age-related changes in autophagy on different aspects of cell biology and organ and system function. The specific aims of the core are: 1) to generate and maintain genetically modified animals with altered autophagy in different tissues; 2) to maintain age-controlled colonies of wild-type and genetically modified mice; 3) to serve as a link among the investigators in the Program Project by coordinating the sacrifice of animals to maximize their use and by integrating the information obtained from the different animal models by each investigator; 4) to facilitate uniformity in the mouse interventions incorporated in all the projects during this new period; 5) to collect life-span information on some of the transgenic mouse models shared by all projects and integrate the heath-span information from each project in the same animals; 6) to work closely with Core B in the characterization of the novel CMA reporter mouse models. The services offered by the Core include: maintenance of the mouse colonies, coordination of genotyping, administration and monitoring of animal treatments (diets and pharmacological compounds), animal dissection, collection of tissue samples for storage or histopathological analysis and continuous access to a searchable animal data base. The key personnel of the Core are the director, who is assisted by the animal technician. The director supervises all the activities of the Core, approves animal use by the PP members, establishes breeding schemes to accommodate the project needs and oversees animal information input in the database. The technician performs all of the activities related to the maintenance of the animal colonies, genotyping, treatments, tissue collection, behavioral testing and inputs information into the database.