Eosinophils, Inflammation and Immunity

我们正在进行的工作主要集中在嗜酸性粒细胞的免疫调节特性上，特别是： 在我们的原始手稿中，我们生成了重组绿色荧光乳杆菌，用于我们正在进行的该制剂与目标嗜酸性粒细胞相互作用的研究。利用Lizier及其同事所描述的pTRKH3p15A-ldhGFP质粒，我们使用8%的甘氨酸/甘油培养基来从我们鉴定的鲁氏乳杆菌免疫生物菌株中创建稳定的转基因培养物。正如这篇手稿所示，我们已经使用这种试剂来探索嗜酸性粒细胞介导的荧光标记细菌的摄取，特别是(但不限于)一种实验室菌株的大肠杆菌，以及革兰氏阳性益生菌，鲁氏乳杆菌L275。这种分析方法还有许多进一步的应用。其中，这一检测方法可以扩展到包括荧光激活细胞分选，以便分离种群，并最终区分携带细菌的嗜酸性粒细胞(即，GFP阳性的)和保持GFP阴性的嗜酸性粒细胞的独特基因表达谱。在此背景下，探索特定基因缺失的影响，特别是与细菌模式和病原体的特定识别有关的影响，也将是耐人寻味的。 引文：Kraemer LS，Brenner TA，Kramholz JO，Rosenberg HF(2017)。一种评价嗜酸性粒细胞介导的益生菌摄取的流式细胞术方法。J.微生物醇。方法137：19-24。 这一周期中的另一篇论文是一篇重要的综述，重点介绍了一些实验，这些实验阐明了CD4+T辅助2型淋巴细胞(TH2细胞)及其相关细胞因子和嗜酸性粒细胞在调节过敏性哮喘特征方面的功能作用。在讨论的结果中，综述强调了最近的证据，即TH2反应的启动受到包括TRAIL和MID1在内的呼吸道上皮细胞衍生因子的调节，同样，microRNAs作为过敏性炎症的关键调节因子和抗炎治疗的潜在靶点所起的作用也是如此。 引文：Foster PS，Maltby S，Rosenberg HF等。(2017)对TH2反应和呼吸道炎症进行建模，以了解调节哮喘发病的基本机制。免疫系统。启示录278：20-40。 我们还为研究界撰写了三篇特邀社论。 引用：Rosenberg HF，Fryer AD，Munitz A，Bochner BS，Jacoby DB，Levi-Schaffer F，Gleich GJ，Furuta GT，Rothenberg ME，Lacy P，Fulkerson PC，Hogan SP，Ackerman SJ，Foster PS(2017)在记忆和庆祝中：James J.Lee临床和实验过敏博士47：980-981 引文：Rosenberg HF，Jacobsen EA，Bochner BS，Weller PF(2017)持久致敬：James J.Lee博士过敏和临床免疫学杂志139：2032 引用：Lyons JJ，Rosenberg HF，Druey Km(2017)通过CRF1强调肥大细胞。《白细胞生物学杂志》，出版中。

Our ongoing work focuses on the immunomodulatory properties of eosinophils, notably: In our original manuscript, we generated recombinant green-fluorescent Lactobacillus for our ongoing studies of the interactions of this agent with target eosinophils. Using the pTRKH3p15A-ldhGFP plasmid with described by Lizier and colleagues, we used an 8% glycine / glycerol medium to create a stable transfectant cultures from our identified immunobiotic strain of L. reuteri. As shown in this manuscript, we have used this reagent to explore eosinophil-mediated uptake of fluorescently-labeled bacteria, notably (but not limited to) a laboratory strain of E. coli, and the Gram-positive probiotic, Lactobacillus reuteri L275. There are numerous further applications of this assay. Among them, this assay can be extended to include fluorescence-activated cell sorting in order to separate populations and ultimately to distinguish unique gene expression profiles characteristic of the eosinophils that have taken up bacteria (ie., that are GFP-positive) compared to those eosinophils that remain GFP-negative. In this context, it will be also intriguing to explore the impact of specific gene-deletions, notably those related to specific recognition of bacterial patterns and pathogens. Citation: Kraemer LS, Brenner TA, Krumholz JO, Rosenberg HF (2017) . A Flow-Cytometric Method to Evaluate Eosinophil-Mediated Uptake of Probiotic Lactobacillus reuteri. J. Microbiol. Methods 137: 19-24. Another publications in this cycle is a major review that highlights experiments that have elucidated functional roles for CD4+ T-helper type-2 lymphocytes (TH 2 cells), their associated cytokines, and eosinophils in the regulation of hallmark features of allergic asthma. Among the findings discussed, the review highlights recent evidence that the initiation of TH2 responses is regulated by airway epithelial cell-derived factors, including TRAIL and MID1, and likewise the roles of microRNAs as key regulators of allergic inflammation and potential targets for anti-inflammatory therapy. Citation: Foster PS, Maltby S, Rosenberg HF et al. (2017) Modeling TH2 responses and airway inflammation to understand fundamental mechanisms regulating the pathogenesis of asthma. Immunol. Rev. 278:20-40. We have also contributed to three invited editorials in service to the research community. Citation: Rosenberg HF, Fryer AD, Munitz A, Bochner BS, Jacoby DB, Levi-Schaffer F, Gleich GJ, Furuta GT, Rothenberg ME, Lacy P, Fulkerson PC, Hogan SP, Ackerman SJ, Foster PS (2017) In memory and celebration: Dr. James J. Lee Clinical and Experimental Allergy 47:980 - 981 Citation: Rosenberg HF, Jacobsen EA, Bochner BS, Weller PF (2017) In lasting tribute: Dr. James J. Lee Journal of Allergy and Clinical Immunology 139:2032 Citation: Lyons JJ, Rosenberg HF, Druey KM (2017) Stressing out mast cells via CRF1. Journal of Leukocyte Biology, in press.