All-Optical Methods for Studying Sequential Motor Behaviors

项目总结 执行已习得的连续运动行为，如演奏已熟知的曲调 在钢琴上，被认为是由大脑中神经元活动的精确序列支持的。然而， 探索顺序神经元活动、神经元连通性和行为之间的联系是具有挑战性的 同时对神经元活动进行空间和时间精确观测和控制的方法。我们 建议应用全光生理学方法，结合并行光遗传操作，以及 群体钙成像，以绘制在经过充分研究的序列中涉及的电路的功能组织 运动行为。这项研究将涉及传播大脑中心的尖端光学方法 主动性和促进更好地理解神经元活动模式是如何隐藏在精确复杂的基础上的 行为是在大脑中产生的。

PROJECT SUMMARY The execution of learned sequential motor behaviors, like those involved in playing a well learned tune on the piano, are thought to be supported by precise sequences of neuronal activity in the brain. However, probing the ties between sequential neuronal activity, neuronal connectivity and behavior is challenging without methods for simultaneously observing and controlling neuronal activity with spatial and temporal precision. We propose to apply all-optical physiological methods, combining concurrent optogenetic manipulation, and population calcium imaging, to map the functional organization of circuits involved in a well-studied sequential motor behavior. This research will involve dissemination of cutting-edge optical methods central to the BRAIN Initiative and facilitate a better understanding of how patterns of neuronal activity underlying precise complex behaviors are generated in the brain.