The human temporal bone laboratory resource for basic and applied inner ear sciences

摘要 在目前的建议中，我们将保存、加工和提供人耳组织标本用于内耳 让基础科学研究人员能够与他们的动物模型进行比较。对.的使用 火棉胶和显微解剖人颞骨组织的免疫组织化学和定量研究 这些技术将有助于推动基础动物内耳研究朝着理解 人类耳部病理，在开发有效的修复药物疗法方面具有很高的潜力 听觉和前庭功能。具体目标1)我们将改善听觉和前庭的通道 研究团体从人的颞骨标本中提取人耳组织。我们将及时完善和推广。 新标本的采购、准备和加工，以及档案的适当再加工 标本。我们将把有限数量的精心准备的实验室组织分发给那些获得资助的研究人员 缺乏足够的人类颞骨准备设施。我们还将利用 关于目前存档的组织及其社区获取信息的登记册，并促进 寻求验证动物模型与人类相关性的研究人员之间的合作。具体目标2) 我们将优化组织制备技术。我们将包括分子和细胞标记以及 可视化技术，允许直接比较新鲜和存档的人体组织与来自 动物模型。我们将发布人耳的采购、保存和制备方案 来自颞骨的组织。通过这样做，我们将鼓励动物研究人员将人类组织纳入 他们的分析。具体目的3)我们将使用高分辨率MRI来评估充满液体的内耳结构， 和体外MRI来评估死后内耳的结构，并与火棉胶进行比较- 嵌入的组织病理学。我们将开发新的技术和方法来形象和可视化人类 中耳和内耳原位，目的是显示软细胞组织、膜和液体以及 硬骨组织。具体目标4)我们将提供技术指导，并提供实践培训 对于研究人员、技术人员耳鼻咽喉科住院医生、医学院和研究生有兴趣 内耳生物学中的颞骨加工技术和人耳加工所需的技能 来自颞骨的组织。这项提议中产生的材料将对研究人员互惠互利 在内耳的基础科学研究中，以及希望应用高水平的 分子和细胞技术应用于人类的颞骨。这些合作努力有望 迅速促进了对颞骨处理的科学研究和对人类耳部病理学的理解。

ABSTRACT In the present proposal we will preserve, process and make available human ear tissue specimens for inner ear basic science researchers such that they may be able to compare with their animal models. The use of celloidin and microdissected human temporal bone tissue for immunohistochemical and quantitative techniques will be instrumental in driving basic animal inner ear research forward towards understanding of human ear pathology and has high potential for the development of effective drug therapies for restoring auditory and vestibular function. In Specific Aim 1) we will improve access for the auditory and vestibular research community to human ear tissues from temporal bone specimens. We will improve and promote timely procurement, preparing and processing of new specimens, and appropriate re-processing of archived specimens. We will distribute a limited number of well-prepared laboratory tissues to funded researchers who lack adequate access to facilities for human temporal bone preparation. We will also exploit use of the Registry for information about currently archived tissues and their access by the community, and promote collaborations between researchers seeking to validate animal models for human relevance. Specific Aim 2) We will optimize tissue preparation techniques. We will include molecular and cellular marking and visualization techniques that allow direct comparisons of fresh and archived human tissues with those from animal models. We will disseminate protocols for procurement, preservation and preparation of human ear tissues from temporal bones. By doing this we will encourage animal researchers to include human tissues into their analyses. Specific Aim 3) We will use high resolution MRI to evaluate the fluid-filled inner ear structures, and ex vivo MRI to evaluate the inner ear structures post-mortem and make comparisons with celloidin- embedded histopathology. We will exploit new technologies and methods to image and visualize human middle and inner ears in situ with the aim of visualizing soft cellular tissues, membranes and fluids, as well as hard bony tissues. Specific Aim 4) We will provide technical instruction with hands-on training will be available for investigators, technicians otolaryngology residents, medical school and graduate students interested in inner ear biology in the temporal bone processing techniques and skills needed for processing human ear tissues from temporal bones. The materials generate in this proposal will be mutually beneficial for researchers in basic scientific studies of the inner ear, as well as to temporal bone scientists wishing to apply high levels of molecular and cellular techniques to the human temporal bone. These collaborative efforts hold promise to rapidly accelerate the science of temporal bone processing and the understanding of human otopathologies.