Centrifugal Precipitation Chromatography: purification of phycoerythrin from crude extract of alge

离心沉淀色谱法已成功地应用于酶、RNA、DNA的纯化。等，使用合适的沉淀剂。 在本研究中，该方法被应用于纯化藻红蛋白的粗提液中使用硫酸铵作为沉淀剂。进行实验以确定最佳硫酸铵梯度（50%-0%饱和度）及其流速（0.5 ml/min）和柱的旋转速度（200 rpm），以在21 h的运行时间内成功纯化目标化合物。 藻红蛋白在色谱图的中间以单峰洗脱出来。 SDS-PAGE分析各峰组分均为单峰，临界吸光度比值均高于参考值。 其他蛋白质如藻蓝蛋白也被分离出来。这些结果可能会在计划于明年夏天在德国举行的第10届国际逆流色谱会议（CCC 2018）上发表。

In the past centrifugal precitation chromatography has been successfully applied to purify enzymes, RNA, DNA. etc. using suitable precipitation agents. In the present research the method was applied for purification of phycoerythrin from a crude extract of algae using ammonium sulfate as a precipitant. The experiments were performed to determine the optimum ammonium sulfate gradient (50%-0% saturation) and its flow rate (0.5 ml/min) and revolution speed (200 rpm) of the column to successfully purify the target compound in 21 h of run time. The phycoerythrin was eluted out at the middle of the chromatogram at a single peak. SDS PAGE analysis of the peak fractions showed a single peak, and critical absorbance ratio was higher than the reference value. Other proteins such as phycocyanin were also separated. These results may be presented at the 10th International Conference on Counter-current Chromatography (CCC2018) which is scheduled in Germany next summer.