Spiral tube assembly for countercurrent chromatography: tubing modification by a simple tool to improve the partition efficincy

采用螺旋管组件的高速逆流色谱可以保留用于蛋白质分离的聚合物相体系中令人满意的数量的固定相。为了提高分配效率，制作了一种简单的油管形状修改工具，并进行了以下四种不同的油管修改：间歇挤压10 mm宽度、平整、平波和平扭。用这些改进的管子制成的分离柱，在800rpm下，用水-水聚合物相系统在1-2ml/min的流速下考察了分离柱的分配效率。结果表明，在流速为1ml/min时，所有改进型管柱的分配效率都有所提高，但在2ml/min的较高流速下，由于保留固定相的损失，扁平管柱的分配效率明显降低。在所有改进柱中，间歇加压管柱的峰分离度最好。结果表明，在半制备分离中，间歇挤压和扁平扭曲可以提高分配效率，而其他改进的扁平和扁波结构的管子可用于低流量的分析分离。

High-speed countercurrent chromatography with a spiral tube assembly can retain a satisfactory amount of stationary phase of polymer phase systems used for protein separation. In order to improve the partition efficiency a simple tool to modify the tubing shapes was fabricated, and the following four different tubing modifications were made: intermittently pressed at 10 mm width, flattened, flat-waving, and flat-twisting. Partition efficiencies of the separation column made from these modified tubing were examined in protein separation with an aqueous-aqueous polymer phase system at flow rates of 1-2 ml/min under 800 rpm. The results indicated that the column with all modified tubing improved the partition efficiency at a flow rate of 1 ml/min, but at a higher flow rate of 2 ml/min the columns made of flattened tubing showed lowered partition efficiency apparently due to the loss of the retained stationary phase. Among all the modified columns, the column with intermittently pressed tubing gave the best peak resolution. It may be concluded that the intermittently pressed and flat-twisting improve the partition efficiency in a semi-preparative separation while other modified tubing of flattened and flat-waving configurations may be used for analytical separations with a low flow rate.