Spiral tube assembly for countercurrent chromatography: tubing modification by a simple tool to improve the partition efficincy

采用螺旋管组件的高速逆流色谱可以保留蛋白质分离所用的聚合物相系统中令人满意的固定相量。 为了提高分配效率，制造了一种简单的工具来修改管材形状，并进行了以下四种不同的管材修改：以10 mm宽度间歇压制、扁平化、扁平波纹和扁平扭曲。 在800 rpm下以1-2 ml/min的流速用水-水聚合物相系统在蛋白质分离中检查由这些改性的管制成的分离柱的分配效率。 结果表明，在流速为1 ml/min时，所有改性管的柱均能提高分配效率，但在较高流速（2 ml/min）时，扁平管的柱由于保留固定相的损失而使分配效率明显降低。在所有改进的色谱柱中，间歇加压色谱柱的峰分离度最好。 可以得出结论，间歇加压和平扭改善分配效率在半制备分离，而其他的扁平和平波配置的修改管道可以用于分析分离与低流速。

High-speed countercurrent chromatography with a spiral tube assembly can retain a satisfactory amount of stationary phase of polymer phase systems used for protein separation. In order to improve the partition efficiency a simple tool to modify the tubing shapes was fabricated, and the following four different tubing modifications were made: intermittently pressed at 10 mm width, flattened, flat-waving, and flat-twisting. Partition efficiencies of the separation column made from these modified tubing were examined in protein separation with an aqueous-aqueous polymer phase system at flow rates of 1-2 ml/min under 800 rpm. The results indicated that the column with all modified tubing improved the partition efficiency at a flow rate of 1 ml/min, but at a higher flow rate of 2 ml/min the columns made of flattened tubing showed lowered partition efficiency apparently due to the loss of the retained stationary phase. Among all the modified columns, the column with intermittently pressed tubing gave the best peak resolution. It may be concluded that the intermittently pressed and flat-twisting improve the partition efficiency in a semi-preparative separation while other modified tubing of flattened and flat-waving configurations may be used for analytical separations with a low flow rate.