Spiral tube assembly for countercurrent chromatography: tubing modification by a simple tool to improve the partition efficincy

高速逆流色谱与螺旋管组件可以保留一个令人满意的数量的固定相的聚合物相系统用于蛋白质分离。为了提高分割效率，制作了一种简单的修改油管形状的工具，并对油管进行了以下四种不同的修改：10 mm宽度的间歇压制、压平、平摇和平扭。在800 rpm下，流速为1-2 ml/min的水-水聚合物相系统中，检测了由这些改性管制成的分离柱的分离效率。结果表明，在流速为1 ml/min时，所有改性管柱的分离效率都有所提高，但在流速为2 ml/min时，由于固定相的损失，扁平管柱的分离效率明显降低。在所有改进柱中，间歇压管柱的峰分辨率最好。可以得出结论，在半制备分离中，间歇压制和平扭管提高了分配效率，而在低流速的分析分离中，可以使用其他扁平和平摇结构的改进管。

High-speed countercurrent chromatography with a spiral tube assembly can retain a satisfactory amount of stationary phase of polymer phase systems used for protein separation. In order to improve the partition efficiency a simple tool to modify the tubing shapes was fabricated, and the following four different tubing modifications were made: intermittently pressed at 10 mm width, flattened, flat-waving, and flat-twisting. Partition efficiencies of the separation column made from these modified tubing were examined in protein separation with an aqueous-aqueous polymer phase system at flow rates of 1-2 ml/min under 800 rpm. The results indicated that the column with all modified tubing improved the partition efficiency at a flow rate of 1 ml/min, but at a higher flow rate of 2 ml/min the columns made of flattened tubing showed lowered partition efficiency apparently due to the loss of the retained stationary phase. Among all the modified columns, the column with intermittently pressed tubing gave the best peak resolution. It may be concluded that the intermittently pressed and flat-twisting improve the partition efficiency in a semi-preparative separation while other modified tubing of flattened and flat-waving configurations may be used for analytical separations with a low flow rate.