Core B: Murine Models and Gnotobiotics Core

小鼠模型已被华盛顿大学消化疾病研究中心广泛使用 中心的研究人员，是中心的DDRCC生物医学研究到消化道的各个方面， 发展、健康和疾病中的系统。小鼠模型和Gnotobiotics核心已经并将 继续主要致力于生产和维持转基因动物，并提供， 为DDRCC成员及时、可靠和具有成本效益地获得和操纵gnotobiotic小鼠 方式此外，Core还为维护小鼠谱系提供辅助服务，例如辅助 繁殖，以及突变或转基因从一种遗传背景到另一种遗传背景的快速转移 使用“速度同源”。我们将在下一个项目期间继续提供这些服务。新的服务以 包括维持一个小鼠银行，将房屋和提供Cre和强力霉素诱导的 转基因小鼠用于DDRCC成员和实施新的，最先进的 产生敲除和敲入小鼠的方法。 本研究的目的包括：（1）研究用转基因、基因敲除和嵌合小鼠的制备和繁殖 消化系统疾病。这包括将DNA注射到受精卵中，将ES细胞注射到囊胚中， 桑椹胚，以及协助育种，殖民地维护，重新推导线作为具体 无病原体和体外受精;（2）特定等位基因在特定菌株背景上的移动， （3）维持含有转基因品系的“小鼠库”， DDRCC研究者;（4）提供未操作的无菌小鼠和基因型匹配的 常规饲养的对照动物;（5）用细菌定殖无菌小鼠;（6） 从现有的常规饲养的转基因小鼠品系中获得无菌小鼠。

Murine models have been extensively utilized by Washington University Digestive Diseases Research Core Center investigators and are central to DDRCC biomedical research into diverse aspects of the digestive system in development, health, and disease. The Murine Models and Gnotobiotics Core has been and will continue to be dedicated primarily to producing and maintaining genetically altered animals and to providing, deriving, and manipulating gnotobiotic mice for DDRCC members in a timely, reliable, and cost-effective manner. In addition, the Core provides ancillary services for maintaining mouse pedigrees, such as assisted reproduction, as well as rapid transfer of mutations or transgenes from one genetic background to another using "speed congenics". We will continue to offer these services in the next project period. New services to be offered include the maintenance of a Mouse Bank that will house and provide Cre and doxycycline-inducible transgenic mice of use to DDRCC members and the implementation of new, state-of-the-art methods for generating knockout and knockin mice. The Aims include: (1) Generation and propagation of transgenic, knockout, and chimeric mice for the study of digestive diseases. This includes injections of DNA into zygotes and of ES cells into blastocysts and morulae, as well as assistance with breeding, colony maintenance, rederivation of lines as specific pathogen-free, and in vitro fertilization; (2) Movement of specific alleles onto defined strain backgrounds by a "speed congenic" approach; (3) Maintenance of a "Mouse Bank" containing transgenic strains of use to DDRCC investigators; (4) Provision of unmanipulated germ-free mice and genotypically matched conventionally-raised control animals; (5) Colonization of germ-free mice with bacteria; (6) Rederivation of germ-free mice from existing conventionally-raised strains of genetically modified mice.