Flow Cytometry Core

项目总结 2011年在桑福德研究中心内成立，作为NIH Cobre支持的一部分(1P20RR024219- 01A2 Grant)癌症生物学研究中心，流式细胞术核心维护和支持众多 设计用于进行多种细胞表型和特征分析的流式细胞仪单元，以及 执行细胞分类/分离协议。Core为其用户提供了各种现代流式细胞仪 应用和分析。Core还配备了装有CellQuest等软件套件的数据分析站， FlowJo和其他公司，并提供所有设备的全面培训。核心目前是额外的 由Sanford Research支持，并积极协助癌症生物学研究促进的项目 中心项目负责人，其他桑福德研究教职员工，以及来自邻近地区的原则研究人员 机构。 凭借Sanford Research对卓越生物医学研究的坚定承诺，以及由此产生的 研究实验室的增长，对流式细胞仪核心服务的需求呈指数级增长 在过去几年里。随着Sanford Research不断扩展到基础生物医学科学领域，因此 我们是否需要最先进的流式细胞仪功能。当前应用程序的目标是 确保核心业务的持续，增强和增强核心的能力，同时 为癌症生物学研究中心提供持续支持。因此，我们建议 流式细胞仪核心将继续为目前Cobre的所有五个项目负责人提供优质服务 续签提案，以及桑福德研究公司的其他调查人员和邻近机构。我们也 打算开发按服务收费的结构，在赠款结束时为Core的自我可持续性提供支持 支持。为实现上述目标，现提出以下具体目标： 1.继续支持Cobre项目负责人和Sanford Research的流式细胞仪需求 教职员工。流式细胞仪核心为调查人员提供现场流式细胞仪服务和支持， 减少动手时间，并允许调查人员和核心工作人员之间进行直接沟通。 2.对新应用进行教育；协助进行有效的实验设计；帮助撰写手稿 准备，并资助申请。核心员工亲自与调查人员及其实验室工作人员就所有 以上科目。 3.加强流式细胞仪核心基础设施。Flow细胞测量核心公司将为其员工增加一名新的 博士后，负责方法论的直接发展和实施 基于Cobre项目具体研究计划的流式细胞仪实验策略 领导，以及监测和实施在流式细胞术方法学的新发展。 4.打造可持续发展的流式细胞术核心。与桑福德研究共享资源委员会联合 我们将开发一种按服务收费的结构，允许Core在Cobre资助期之后的可持续性。

PROJECT SUMMARY Established within the Sanford Research in 2011 as a part of NIH COBRE-supported (1P20RR024219- 01A2 grant) Cancer Biology Research Center, the Flow Cytometry Core maintains and supports numerous flow cytometric units designed to perform multiple cell phenotyping and characterization analyses, as well as to execute cell sorting/separation protocols. The Core delivers to its users a variety of modern flow cytometry applications and analyses. The Core also houses data analysis stations with software suits such as CellQuest, FlowJo and others, and offers comprehensive training on all its equipment. The Core is currently additionally supported by Sanford Research, and actively assists with the projects facilitated by Cancer Biology Research Center Project Leaders, other Sanford Research faculty, and by principle investigators from neighboring institutions. With Sanford Research's strong commitment to excellence in biomedical research and a consequent growth in research laboratories, the demand for Flow Cytometry Core services has exponentially growing over the last several years. As Sanford Research continues to expand into areas of basic biomedical sciences, so does our need for the state-of-the-art flow cytometry capabilities. The objective of the current application is to secure the continuation of the Core's operations, to augment and enhance Core's capabilities, while providing continuous support of the Cancer Biology Research Center. Accordingly, we propose that the Flow Cytometry Core will continue delivering quality services to all five Project Leaders of the current COBRE renewal proposal, as well as other investigators at Sanford Research, and neighboring institutions. We also intent to develop a fee-for service structure, providing for Core's self-sustainability at the end of the grant support. To achieve the above-stated objective, the following Specific Aims are proposed: 1. To continue support the flow cytometric needs of the COBRE Project Leaders and Sanford Research faculty. The Flow Cytometry Core provides investigators with on-site flow cytometry services and support, decreasing hands-on time and allowing for direct communication between investigators and Core staff. 2. To educate on novel applications; assist with efficient experimental design; help with manuscript preparation, and funding applications. Core staff personally interacts with investigators and their lab staff on all above subjects. 3. To enhance the Flow Cytometry Core infrastructure. The Flow Cytometry Core will add to its staff a new Postdoctoral Fellow who will be responsible for the direct methodological development, and implementation of flow cytometry-based experimental strategies pertinent to the specific research plans of COBRE Project Leaders, as well as for monitoring and implementing a new developments in flow cytometric methoodology. 4. To create a sustainable Flow Cytometry Core. Jointly with Sanford Research Shared Resources Committee we will develop a fee-for-service structure, allowing Core's sustainability beyond the COBRE funding period.