Core B _BIDMC

核心B总结 分子生物学核心（核心B）将提供专家和高效的实验室服务 项目1（Saper）、项目2（Scammell）和项目4（Chamberlin）。主要职责 核心B是小鼠集落的管理和基因分型， 杂交组织化学核心B还将管理细菌克隆， 杂交和病毒载体，并产生新/修饰的DNA构建体作为技术 进化 Core B旨在优化功效、准确性和专业知识。核心将管理 大型共享小鼠群，以确保可靠和有效地提供准确的 每个项目的基因分型小鼠。核心还将集中性能 复杂的分子技术，如原位杂交组织化学和细菌 这样项目中的研究人员就可以专注于科学而不是调试 技术.我们多年的经验与这些方法最大的可能性， 本PPG中提出的复杂分子技术将获得成功。

Summary of Core B The Molecular Biology Core (Core B) will provide expert and efficient laboratory services to Project 1 (Saper), Project 2 (Scammell) and Project 4 (Chamberlin). The major duties of Core B are management and genotyping of mouse colonies and performing in situ hybridization histochemistry. Core B will also manage bacterial clones for in situ hybridization and viral vectors, and produce new/modified DNA construct as technology evolves. Core B is designed to optimize efficacy, accuracy and expertise. The Core will manage large, shared mouse colonies to ensure a reliable and efficient supply of accurately genotyped mice for each Project. The Core also will centralize the performance of complex molecular techniques such as in situ hybridization histochemistry and bacterial culture so researchers in the Projects can focus on the science instead of debugging techniques. Our years of experience with these methods maximizes the likelihood that the complicated molecular techniques proposed in this PPG will succeed.