Silk-based biospecimen stabilization for exposure assessment diagnostics

用于暴露评估的丝基生物有机胺稳定剂 项目摘要/摘要 迫切需要一种稳定平台，能够保持生物样品的完整性，完全不受 冷藏要求。这种技术将使研究人员、临床医生、政府人员和 消费者在现场条件下进行生物监测(即不需要访问门诊实验室)。我们的 长期目标是开发一种平台技术，最大限度地提高各种生物谱系的稳定性 在一种新型的丝蛋白基质中，并与下游分析无缝集成。该计划的目标是 目前的建议是建立一种新型的收集设备，能够捕获已知体积的样本，集成 在我们的第一阶段计划中研究的丝绸基质稳定剂，并允许立即存储和 装运时采用干燥剂干燥。我们的中心假设是可以设计一种新的收集装置 为了克服当前样品的实用设计和基本技术限制 收藏选项。我们的假设是基于我们最近发布和未发布的数据而形成的 使用丝素稳定一系列血清/血浆蛋白，并对加工技术进行新的洞察，从而使 丝绸应在高温下长期储存和运输。因此，在第一个目标中，我们将 开发一种新颖的收集装置，以适应新的丝绸功能。作为第二个目标，一个50人的队列 将采集的静脉血、毛细血管血和唾液样本，进行均分并储存在丝绸中 系统、干纸或作为液体等分受到各种处理延迟的影响，以模拟远程运输 用例。在第三个目标中，将评估从现有印度队列中提取的200名受试者的 危险暴露(Vaxess)和血浆肝、甲状腺和心血管疾病生物标记物水平(Agada 医学)。预期结果将是丝绸稳定和新的 方法将这些发现转化为与生物监测有关的生物样品回收和储存。 这项拟议研究的基本原理是，一旦收集设备得到验证，它们就可以用于 各种外部合作伙伴关系设置。计划方法的成功可以对以下许多领域产生积极影响 在诊断学和流行病学领域的需要。这些需求包括Solid的替代方法 生物胺稳定化，以及对危险暴露非常重要的分析技术的翻译/验证。 此外，通过提供与整体的指刺量(即侵入性较小的量)相接口的方法 血液，一个以丝绸为基础的稳定系统可以显著减轻与 门诊，反过来又增加了可达性。

Silk-Based Biospecimen Stabilization for Exposure Assessment Project Summary/Abstract There is a critical need for a stabilization platform that can preserve biospecimen integrity, completely free of cold storage requirements. Such a technology would allow researchers, clinicians, government personnel and consumers to conduct biomonitoring in field conditions (i.e. without requiring visits to the outpatient lab). Our long-term goal is to develop a platform technology that maximizes the stability of a wide range of biospecimens in a novel silk protein matrix and that seamlessly integrates with downstream analytics. The objective of the current proposal is to build a novel collection device capable of capturing known volumes of specimen, integrating with the silk matrix stabilizer investigated in our Phase I program, and allowing for immediate storage and shipment via the use of desiccant drying. Our central hypothesis is that a new collection device can be designed in order to overcome both practical design-oriented and fundamental technological limitations of current sample collection options. Our hypothesis has been formulated based on our recent published and unpublished data using silk to stabilize a range of serum/plasma proteins and new insights into processing techniques that allow silk to be stored and shipped for extended durations at elevated temperatures. Therefore, in the first aim we will develop a novel collection device to accommodate the new silk feature. As a second aim, a 50-person cohort will be recruited for venous blood, capillary blood, and saliva samples, to be aliquoted and stored in the silk system, dried paper, or as liquid aliquots subject to various processing delays to simulate remote transportation use cases. In the third aim, two hundred subjects taken from an existing Indian cohort will be evaluated for hazard exposure (Vaxess) and plasma levels of liver, thyroid, and cardiovascular disease biomarkers (Agada medical). The expected outcomes would be a broader fundamental basis behind silk stabilization and new methods to translate these findings towards biospecimen recovery and storage as they pertain to biomonitoring. The rationale for the proposed research is that, once collection devices are validated, they can be used in a variety of external partnership settings. Success with the planned approach can positively impact many areas of need in the field of diagnostics and epidemiology. These needs include alternative approaches at solid biospecimen stabilization, and translation/validation of analytical techniques important for hazard exposures. Furthermore, by offering methods to interface with finger-prick volumes (i.e. less invasive quantities) of whole blood, a silk-based stabilization system could significantly reduce financial burdens associated with the outpatient clinic, and in turn increase accessibility.