Core B: Integrated Microscopy Core

摘要 研究核心的总体目标是提高研究人员阐明感觉系统如何 工作以及疾病/病理状态如何发生。为此，我们建议加强和扩大 通过创建集成显微镜核心（IMcore）来增强现有显微镜核心的功能。IMcore是 建立在三个主要服务组件上：1）显微镜核心，2）抗体单元（Au）和3）分子 分析股。所有组件将在物理和主题上集成到现有的显微镜中 核心现任国家资助的主任张兆杰博士领导建立了 显微镜核心作为一个关键的机构设施，将继续在这一角色作为IMcore主任。一个多- 一个由科学家和技术人员组成的学科小组将管理这三个组成部分的日常运作。 提出了两个具体目标。目标1。为了更新和扩展现有的显微镜核心以创建IMcore， 并提供一个平台，以促进必要的合作和培训，以满足成像的需要， investigators.要绘制出大面积的图像，需要一种高速、高分辨率的多光子显微镜 在体内和体外的三维感觉网络。目标2.为了扩大服务能力， IMcore通过提供抗体筛选和开发以及分子分析能力。我们将扩大 现有核心包括抗体开发和验证。抗体的验证使用各种 分子技术包括蛋白质印迹、免疫沉淀、敲除动物和PCR， 给蛋白质的信息服务和仪器，使新的国家的最先进的高， 通量“分子成像”（例如，ChIP-seq，RNA-seq）的神经系统，部分利用外部的fee- 将开发下一代服务设施。当前显微镜核心的成功扩展 将整合新的能力，将：1）满足未来的技术挑战，使创新研究， SBC调查员，2）促进SBC调查员之间新的和更强有力的合作，并在这样做的过程中，3） 为SBC调查人员采取协作和开创性的方法来了解 感觉系统如何工作以及疾病/病理状态如何发生。

Abstract The overall goal of the research core is to enhance the ability of researchers to elucidate how sensory systems work and how disease/pathological states occur. To do so, we propose to strengthen and expand the capabilities of the existing Microscopy Core by creating an Integrated Microscopy Core (IMcore). The IMcore is built on the three main service components.1) Microscopy Core, 2) the Antibody Unit (AU), and 3) Molecular Analysis Unit (MAU). All components will be integrated physically and thematically into the existing Microscopy Core. Dr. Zhaojie Zhang, the current state-funded director, who has provided the leadership to establish the Microscopy Core as a critical institutional facility, will continue in this role as Director of the IMcore. A multi- disciplinary team of scientists and technicians will manage the day-to-day operation of the three components. Two specific aims are proposed. Aim 1. To update and expand the existing microscopy core to create IMcore, and to provide a platform to promote collaborations and training necessary to meet the imaging needs of the investigators. A multi-photon microscope with high speed and high resolution is needed to map out large sensory network in three dimensions in vivo and in vitro. Aim 2. To expand the service capabilities of the IMcore by providing antibody screening and development and molecular analytic capabilities. We will expand the existing core to include antibody development and validation. The validation of antibodies uses a variety of molecular techniques including western blotting, immunoprecipitation, knock-out animals, and PCR to relate message to proteins. Services and instruments enabling the application of the new state-of-the-art high- throughput “molecular imaging” (e.g., ChIP-seq, RNA-seq) of the nervous system utilizing, in part, outside fee- for-service next generation facilities will be developed. Successful expansion of the current Microscopy Core will integrate new capacities that will: 1) meet future technical challenges and enable innovative research by SBC investigators, 2) foster new and stronger collaborations among SBC investigators and in doing so, 3) pave the way for SBC investigators to undertake collaborative and groundbreaking approaches to understand how sensory systems work and how disease/pathological states occur.