Pathobiology and biomechanics of vocal fold dehydration

项目总结 许多该领域的专家认为，为了保持嗓音健康，最佳的身体水合是必不可少的。对这件事 最后，通常建议尽量减少脱水和增加水分，以防止声音问题和AS 正在进行的问题的治疗。这一建议背后的假设是，脱水会导致 声带的不利病理和生物力学变化，扬声器将会发育 适应不良，声音创伤的行为来补偿。由于声音创伤嗓音的盛行 问题是，开始补水治疗已经成为一种常见的、毫无根据的临床建议。这个 在这一论点中，关键的、缺失的一环是缺乏数据表明不利的病理生物学和 脱水的生物力学效应，以及测试这些数据端点的现实模型。一个逼真的模型 应具有调节体内水合作用的完整的动态平衡机制，并服从严格的 水化状态的控制。一个脱水的兔动物模型系统地满足了这些要求。 这项建议的第一个目标是量化急性胰腺炎的不良病理生物学和生物力学后遗症。 脱水。全身和体表脱水将被诱导到与生理相关的水平， 已被证明可以引起人类说话者的声音变化。系统性和表面性的明显影响 将对幼兔和老年兔的脱水进行量化，以描绘不同途径的影响 脱水，以及年龄，对液体稳态的影响。这项提案的第二个目标是确定 全身和体表复水可逆转青少年脱水所致的不良声带改变 还有老兔子。该提案的第三个目标是建立慢性系统性和慢性的不同影响 幼兔和老年兔的表面脱水。慢性脱水可能不仅会增加 在急性脱水中观察到，但也可能揭示第一次未发现的退化机制 两个目标。病理生物学和生物力学终点将包括质子密度磁共振成像作为一种非侵入性 声带水化状态的测量，以及组织学、细胞、分子和生物力学评估 脱水和复水后的声带皱折。通过结合细胞、分子、结构和 功能技术，这项全面的提案试图从根本上洞察 改变声带液、上皮、固有层和肌肉组织的水合状态，从而验证 可以对脱水的不利影响和治疗益处提出科学建议 水合作用。

PROJECT SUMMARY Optimal body hydration is considered imperative to preserving vocal health by many experts in the field. To this end, minimizing dehydration and increasing hydration is often recommended to prevent voice problems and as treatment for ongoing problems. The presumption underlying this recommendation is that dehydration induces adverse pathological and biomechanical changes to the vocal folds, and that speakers will develop maladaptive, phonotraumatic behaviors to compensate. Due to the prevalence of phonotraumatic voice problems, initiating hydration treatments has become a common, unfounded clinical recommendation. The critical, missing links in this argument are the absence of data demonstrating adverse pathobiological and biomechanical effects of dehydration, and a realistic model to test these data endpoints. A realistic model should have intact homeostatic mechanisms that regulate hydration in vivo and be amenable to rigorous control of hydration state. A rabbit animal model that is dehydrated systematically meets these requirements. The first goal of this proposal is to quantify the adverse pathobiological and biomechanical sequelae of acute dehydration. Systemic and surface dehydration will be induced to physiologically-relevant levels that have been shown to induce voice changes in human speakers. The distinct effects of systemic and surface dehydration will be quantified in young and old rabbits in order to delineate the influence of route of dehydration, as well as age, on fluid homeostasis. The second goal of this proposal is to establish whether systemic and surface rehydration can reverse adverse vocal fold changes induced by dehydration in young and old rabbits. The third goal of this proposal is to establish differential effects of chronic systemic and chronic surface dehydration in young and old rabbits. Chronic dehydration might not only increase impairments that are observed in acute dehydration, but may also reveal mechanisms of degeneration not identified in the first two goals. Pathobiological and biomechanical endpoints will include proton density MRI as a non-invasive measure of vocal fold hydration state, and histological, cellular, molecular, and biomechanical assessment of the vocal folds following dehydration and rehydration. By combining cellular, molecular, structural, and functional techniques, this comprehensive proposal seeks to shed fundamental insight into the effects of altered hydration state on vocal fold fluid, epithelium, lamina propria and muscle tissue so that validated scientific recommendations can be made on the adverse effects of dehydration and the therapeutic benefits of hydration.