Strategies and Techniques for Analyzing Microbial Population Structures

描述（由申请人提供）：分析微生物种群结构的策略和技术（STAMPS）-伍兹霍尔海洋生物实验室的研讨会。该提案描述了一个新的，为期10天的再现课程，将侧重于设计和分析标记基因和鸟枪宏基因组项目，为高级研究生，博士后研究员和既定的研究人员寻求利用并行DNA测序技术的力量来调查微生物群落，包括人类微生物和宿主相关微生物。该格式将包括一系列讲座和计算机实验室练习的理论，应用和实验策略，通过大规模并行，高通量DNA测序技术分析微生物群落。主题将包括：DNA和RNA的大规模并行测序（MPS）的当前技术，在微生物群落研究中使用MPS的实验策略，文库制备技术，下一代序列数据和相关元数据的组织，序列数据的质量控制原理，通过相似性和稀疏分析聚类序列的理论，高通量数据的分类学分配，用于估计微生物多样性的统计模型、微生物群落比较方法和指标、网络分析以及鸟枪宏基因组数据的注释和解释。分析标记基因和鸟枪宏基因组数据的分析工具的作者将用教程挑战学生，这些教程解决了影响人类健康和福祉的真实的世界问题。使用他们的笔记本电脑，学生将获得访问的高性能计算机架构在约瑟芬湾保罗中心在MBL的大型数据集的分析。同样重要的是，这门新课程将促进专家之间的对话和思想交流，这些专家寻求为快速发展的分子微生物生态学领域指导新的分析范式的发展。在课程安排的早期，学生将有机会展示研究海报，以促进与其他学生和教师的互动，他们可以提供有关数据分析的建议。

DESCRIPTION (provided by applicant): Strategies and Techniques for Analyzing Microbial Population Structures (STAMPS) - A workshop at the Marine Biological Laboratory in Woods Hole. This proposal describes a new, 10-day emersion course that will focus on design and analysis of marker gene and shotgun metagenomic projects for advanced graduate students, postdoctoral fellows and established investigators who seek to leverage the power of massively-parallel DNA sequencing technologies for investigations of microbial communities including the human microboime and host associated microbes. The format will include a series of lectures and computer laboratory exercises on the theory, application and experimental strategies for analysis of microbial communities through massively parallel, high-throughput DNA sequencing technology. Topics will include: current technologies for massively parallel sequencing (MPS) of DNA and RNA, experimental strategies for using MPS in studies of microbial communities, library preparation techniques, organization of next generation sequence data and associated meta-data, principles of quality control of sequence data, the theory of clustering sequences by similarity and rarefaction analyses, taxonomic assignments for high-throughput data, statistical models for estimating microbial diversity, microbial community comparison methodology and metrics, network analyses, and the annotation and interpretation of shotgun metagenomic data. Authors of analytical tools for analysis of marker gene and shotgun metagenomic data will challenge the students with tutorials that address real world problems that bear on human health and well- being. Using their laptops, students will gain access to the high-performance computer architecture in the Josephine Bay Paul Center at the MBL for analysis of large data sets. As importantly, this new course will promote a dialogue and exchange of ideas between experts who seek to guide the development of new analytical paradigms for the rapidly evolving field of molecular microbial ecology. Early in the course schedule, students will have the opportunity to present research posters in order to promote interactions with other students and with faculty who can offer advice about data analysis.