Core B: Anatomy and Behavior Core

总结-核心B 解剖学和非人类灵长类动物行为核心（核心B）将是尤德尔中心的重要组成部分， 埃默里大学。这个核心的主要功能之一将是准备和处理脑组织， 实验动物由中心项目产生。这项服务将通过标准化的组织学检查完成。 技术，并将提供信息，这将是必不可少的解释功能 项目1和2下的试验，并将促进所有三个项目之间的成果分享和比较 项目具体而言，核心将提供以下服务：（1）使用免疫组化和 显微镜方法来评价用6-羟色胺处理的动物中多巴胺能耗竭的程度。 羟基多巴胺（6-OHDA，在项目1中用于小鼠）或1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP， 在项目2和3中用于猴子）;（2）使用组织学标记物描绘运动丘脑中的核 啮齿动物（项目1）和猴子（项目2和3）;（3）记录电极的组织学重建 轨迹（项目1和2）;（4）病毒感染后视蛋白表达的程度和模式的分析 转染（项目1和2）;（5）充满生物细胞素的神经元的检测和数字重建（项目1）;以及 (6)，MPTP处理的猴子的脑组织的编目和存储。核心B的第二个主要服务 将用神经毒素MPTP对项目2和项目3中的恒河猴进行系统治疗， 用标准化方法进行这种治疗而导致的帕金森症。这将使我们能够产生动物 在两个项目中，帕金森症的水平相似，这将有助于比较 他们通过提供这些服务，核心将有助于从研究项目中腾出时间和资源。 此外，使用标准化的解剖和行为评估技术将有助于我们保持 中心研究的一贯高质量水平。

Summary – Core B The Anatomy and non-human primate behavior Core (Core B) will be an essential part of the Udall Center at Emory University. One of the major functions of this Core will be to prepare and process brain tissue from experimental animals generated by the Center projects. This service will be done with standardized histologic techniques, and will provide information that will be indispensable for the interpretation of the functional experiments under projects 1 and 2, and will facilitate sharing and comparisons of results among all three projects. Specifically, the Core will provide the following services: (1) Use of immunohistochemical and microscopy approaches to evaluate the degree of dopaminergic depletion in animals treated with 6- hydroxydopamine (6-OHDA, used in mice in project 1) or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, used in monkeys in projects 2 and 3); (2) use of histological markers to delineate nuclei in the motor thalamus in rodents (projects 1) and monkeys (projects 2 and 3); (3) histological reconstructions of recording electrode trajectories (projects 1 and 2); (4) analysis of the degree and pattern of expression of opsins after viral transfection (projects 1 and 2); (5) detection and digital reconstruction of biocytin-filled neurons (project 1); and (6), the cataloguing and storing of brain tissue of MPTP-treated monkeys. A second major service of Core B will be to systemically treat Rhesus monkeys from projects 2 and 3 with the neurotoxin MPTP and evaluate parkinsonism resulting from this treatment with standardized methods. This will allow us to generate animals with similar levels of parkinsonism across both projects, and will facilitate comparisons of data generated by them. By providing these services, the Core will help to free up time and resources from the research projects. In addition, the use of standardized anatomical and behavioral assessment techniques will help us to maintain a consistently high level of quality of Center research.