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SYNPLA: A scaleable method for monitoring circuit-specific learning-induced changes in synaptic strength

SYNPLA: A scaleable method for monitoring circuit-specific learning-induced changes in synaptic strength
SYNPLA：一种可扩展的方法，用于监测特定电路学习引起的突触强度变化
批准号：
9301350
负责人：
ROBERTO MALINOW
金额：
$92.47万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
项目状态：
已结题
起止时间：
2015-09-25 至 2019-06-30
来源：
https://reporter.nih.gov/project-details/9301350
关键词：
AMPA ReceptorsAmygdaloid structureAnimalsAntibodiesAuditoryAutistic DisorderBehaviorBiochemicalBiological AssayBrainBrain regionCorpus striatum structureDNADetectionDiseaseElectron MicroscopyElectrophysiology (science)EnsureExcitatory SynapseGlutamate ReceptorGlutamatesGoalsHabenulaHippocampus (Brain)ImageIn SituIn VitroIndividualLaboratoriesLateralLearningLigationLong-Term PotentiationMediatingMemoryMental DepressionMethodsModernizationMolecularMonitorNeuraxisNeuronsNeurosciencesNucleotidesNucleus AccumbensOligonucleotidesPlayProteinsResolutionSignal TransductionSliceSpecificityStimulusSynapsesSynaptic plasticityTestingTimeValidationVentral Tegmental AreaWorkaddictionbasebehavioral plasticityexperiencein vivoneuropsychiatric disordernovelpostsynapticpresynapticpublic health relevancereceptortooltrafficking

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中文摘要
翻译
 描述（由申请人提供）：本申请的目标是开发SYNPLA，一种特异性、选择性和高通量的方法，用于标记具有单突触分辨率的经验诱导的可塑性。SYNPLA利用了我们对长时程增强的分子机制的理解，长时程增强是通过在最近增强的突触处插入谷氨酸受体的特定亚型来介导的。在目标1中，我们在培养的神经元中开发SYNPLA。在目标2和3中，我们将其应用于两个脑回路，皮质-杏仁核和皮质-纹状体，这两个脑回路分别被称为经历经验依赖性可塑性。SYNPLA有可能成为一个强大的工具，解剖电路机制的行为可塑性。
英文摘要
 DESCRIPTION (provided by applicant): The goal of this application is to develop SYNPLA, a specific, selective and high-throughput method for marking experience-induced plasticity with single synapse resolution. SYNPLA exploits our understanding of the molecular mechanism of long-term potentiation, which is mediated by the insertion of a specific subtype of glutamate receptor at recently potentiated synapses. In Aim 1 we develop SYNPLA in cultured neurons. In Aims 2 and 3 we apply it to two brain circuits, cortico-amygdalar and cortico-striatal respectively which are known to undergo experience dependent plasticity. SYNPLA has the potential to emerge as a powerful tool for dissecting the circuit mechanisms underlying behavioral plasticity.
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SYNPLA: A scaleable method for monitoring circuit-specific learning-induced changes in synaptic strength
  • 批准号：
    9037290
  • 项目类别：
  • 资助金额：
    $100.59万
  • 财政年份：
    2015
  • 负责人：
    ROBERTO MALINOW
  • 依托单位：
    COLD SPRING HARBOR LABORATORY
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