Control of AMPA Receptor Trafficking by Beta Amyloid

Beta 淀粉样蛋白控制 AMPA 受体运输

• 批准号: 8042654
• 负责人: ROBERTO MALINOW
• 金额: $ 51.99万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8042654
• 关键词: AMPA Receptors; Affect; Alzheimer' s Disease; Amyloid beta-Protein; Biochemical; Cessation of life; Complement; Cytoplasmic Tail; Dementia; Dendritic Spines; Deposition; Disease; Elderly; Electron Microscopy; Electrophysiology (science); Etiology; Event; Excision; Functional disorder; Glutamate Receptor; Grant; Hippocampus (Brain); Impaired cognition; Laser Scanning Microscopy; Long-Term Potentiation; Memory; Mental Depression; Methodology; Modeling; Modification; Molecular; Molecular Biology; N-Methyl-D-Aspartate Receptors; N-Methylaspartate; Neurodegenerative Disorders; Neurofibrillary Tangles; Neurons; Pathogenesis; Pathologic; Peptides; Phosphorylation; Phosphotransferases; Play; Price; Process; Property; Protein Kinase; Protein Kinase C; Protein phosphatase; Rattus; Recruitment Activity; Role; Series; Signal Transduction; Slice; Synapses; Synaptic Transmission; Synaptic plasticity; Testing; Transgenic Mice; Vertebral column; Whole-Cell Recordings; calmodulin-dependent protein kinase II; conditioning; extracellular; neurotoxic; patch clamp; relating to nervous system; therapeutic target; trafficking; treatment strategy; two-photon

DESCRIPTION (provided by applicant): Long-term potentiation and depression (LTP and LTD) are promising and widely studied examples of vertebrate synaptic plasticity. In LTP and LTD there is a persistent synaptic enhancement or decrement, respectively, seen following brief conditioning periods of synaptic activity. In both these forms of plasticity, which are leading models of memory, the trafficking of AMPA receptors (-Rs) at synapses plays a key role. The general aim of this grant has been to examine the subcellular signaling controlling AMPA-R trafficking. Recently, we have found that beta amyloid (A?), a peptide strongly implicated as a causative agent in Alzheimer's disease, has pronounced effects on AMPA-R trafficking. In this grant period, we will examine how A? can control the trafficking of synaptic AMPA receptors. Our recent studies show that A? recruits signaling used in LTD to remove synaptic AMPA receptors. Furthermore, loss of synaptic AMPA receptors leads to loss of dendritic spines and NMDA receptors; that is, loss of the synapse. Here we will determine the mechanisms by which A? leads to these events. Several complementing methodologies will be used, including molecular biology, electrophysiology, two-photon laser scanning microscopy, and electron microscopy. These studies will use organotypic rat hippocampal slices, dissociated cultured neurons and transgenic mice. The results of these studies will elucidate the mechanisms underlying Alzheimer's disease as well as provide potentially efficacious treatment strategies. The specific aims are to determine: SA1: If A? allosterically up-modulates NMDA-R function; SA2: How A? interacts with synaptic plasticity; SA3: How A? leads to removal of synaptic AMPA-Rs; SA4: How A? leads to loss of synapses. There is growing evidence that one of the first targets of dysfunction in Alzheimer's disease is the synapse. We will examine the mechanisms by which beta amyloid, a molecule strongly implicated in the etiology of the disease, leads to synaptic dysfunction. By elucidating these mechanisms we will identify potentially therapeutic targets in the treatment of Alzheimer's disease.

描述（由申请人提供）：长时程增强和抑制（LTP和LTD）是脊椎动物突触可塑性的有前途和广泛研究的例子。在LTP和LTD中，在短暂的突触活动调节期之后，分别观察到持续的突触增强或减少。在这两种形式的可塑性中，这是记忆的主要模型，突触上AMPA受体（-Rs）的运输起着关键作用。这项资助的总体目标是研究控制AMPA-R运输的亚细胞信号。最近，我们发现β淀粉样蛋白（A？），一种强烈暗示为阿尔茨海默病病原体的肽，对AMPA-R运输具有显著影响。 在这段时间内，我们将研究如何A？可以控制突触AMPA受体的运输。我们最近的研究表明，A？招募LTD中使用的信号以移除突触AMPA受体。此外，突触AMPA受体的丧失导致树突棘和NMDA受体的丧失;即，突触的丧失。在这里，我们将确定的机制，A？导致了这些事件。将使用几种补充方法，包括分子生物学，电生理学，双光子激光扫描显微镜和电子显微镜。这些研究将使用器官型大鼠海马切片、分离培养的神经元和转基因小鼠。这些研究的结果将阐明阿尔茨海默病的潜在机制，并提供潜在有效的治疗策略。具体目标是确定：SA 1：如果A？变构上调NMDA-R功能; SA 2：如何A？与突触可塑性相互作用; SA 3：如何A？导致突触AMPA-R的去除; SA 4：如何A？导致突触的丧失 越来越多的证据表明，阿尔茨海默病功能障碍的首要目标之一是突触。我们将研究β淀粉样蛋白（一种与疾病病因密切相关的分子）导致突触功能障碍的机制。通过阐明这些机制，我们将确定潜在的治疗阿尔茨海默病的治疗目标。