SYNPLA: A scaleable method for monitoring circuit-specific learning-induced changes in synaptic strength

SYNPLA：一种可扩展的方法，用于监测特定电路学习引起的突触强度变化

• 批准号: 9037290
• 负责人: ROBERTO MALINOW
• 金额: $ 100.59万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-25 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9037290
• 关键词: AMPA Receptors; Amygdaloid structure; Animals; Antibodies; Auditory; Autistic Disorder; Behavior; Behavioral; Biochemical; Biological Assay; Brain; Brain Diseases; Brain Part; Brain region; Corpus striatum structure; DNA; Detection; Disease; Electron Microscopy; Electrophysiology (science); Ensure; Excitatory Synapse; Glutamate Receptor; Glutamates; Goals; Habenula; Hippocampus (Brain); Image; In Situ; In Vitro; Individual; Laboratories; Lateral; Learning; Ligation; Long-Term Potentiation; Mediating; Memory; Mental Depression; Methods; Molecular; Monitor; Neuraxis; Neurons; Neurosciences; Nucleotides; Nucleus Accumbens; Oligonucleotides; Play; Proteins; Resolution; Signal Transduction; Slice; Specificity; Stimulus; Synapses; Synaptic plasticity; Testing; Time; Validation; Ventral Tegmental Area; Work; addiction; base; experience; in vivo; neuropsychiatry; novel; postsynaptic; presynaptic; public health relevance; receptor; tool; trafficking

 DESCRIPTION (provided by applicant): The goal of this application is to develop SYNPLA, a specific, selective and high-throughput method for marking experience-induced plasticity with single synapse resolution. SYNPLA exploits our understanding of the molecular mechanism of long-term potentiation, which is mediated by the insertion of a specific subtype of glutamate receptor at recently potentiated synapses. In Aim 1 we develop SYNPLA in cultured neurons. In Aims 2 and 3 we apply it to two brain circuits, cortico-amygdalar and cortico-striatal respectively which are known to undergo experience dependent plasticity. SYNPLA has the potential to emerge as a powerful tool for dissecting the circuit mechanisms underlying behavioral plasticity.

 描述(由申请人提供)：本申请的目标是开发SYNPLA，一种特异、选择性和高通量的方法，用于标记单一突触分辨率的经验诱导可塑性。SYNPLA利用了我们对长期增强的分子机制的理解，长期增强的分子机制是通过在最近增强的突触插入特定亚型的谷氨酸受体来介导的。在目标1中，我们在培养的神经元中发展了SYNPLA。在目标2和目标3中，我们将其应用于两个大脑回路，分别是杏仁皮质和纹状体，已知这两个回路经历了经验依赖性的可塑性。SYNPLA有可能成为剖析行为可塑性背后的回路机制的有力工具。