Perkin Elmer Sciclone G3 NGSx Workstation for High Throughput Sequencing Library Preparation

摘要 维克森林浸信会综合癌症中心（WFBCCC）癌症基因组学核心实验室， NCI支持的共享资源正在申请资金以购买Perkin Elmer Sciclone G3 NGSx Perkin Elmer工作站该工作站是一个自动化平台，旨在为 下一代DNA测序（NGS）平台。该仪器设计灵活，具有一定的测量能力， 以每次运行制备8-96个文库。Sciclone G3 NGSx工作站为所有NGS准备文库 应用程序包括RNAseq，ChIPseq，DNAseq，外显子组测序和小RNAseq，并附带 预设程序，用于从多个供应商制备NGS库。该工作站具有用户友好的 可编程功率控制器，可编程以按原样实施新的NGS库协议 开发该仪器将位于癌症基因组学核心实验室，该实验室由 WFBCCC共享资源授权。该工作站将支持10个NIH资助的主要用户和6个次要用户 在维克森林大学健康科学（WFUHS）的多个部门获得NIH赠款的用户。这 仪器赠款有杰出的支持，从WFUHS教师和杰出的财政支持， WFUHS和WFBCCC部门主席和管理

Abstract The Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) Cancer Genomics Core Laboratory, an NCI-supported Shared Resource is requesting funds to purchase a Perkin Elmer Sciclone G3 NGSx Workstation from Perkin Elmer. This workstation is an automated platform designed to prepare libraries for Next Generation DNA Sequencing (NGS) platforms. The instrument is flexible in design and has the capability to prepare 8-96 libraries per run. This Sciclone G3 NGSx Workstation prepares libraries for all NGS applications including RNAseq, ChIPseq, DNAseq, exome sequencing, and small RNAseq, and comes with preset programs for NGS libraries preparation from multiple vendors. The workstation has a user friendly programmable power controller that can be programmed to implement new NGS library protocols as they are developed. The instrument will be located in the Cancer Genomics Core Laboratory, which is supported by the WFBCCC Shared Resource Grant. The workstation will support 10 Major Users with NIH funding and 6 Minor Users with NIH grants across multiple departments at Wake Forest University Health Sciences (WFUHS). This instrumentation grant has outstanding support from the WFUHS faculty and outstanding financial support from the WFUHS and WFBCCC department chairs and administration