Biomedical Research Core 2: Epigenetics Core

项目摘要 生物医学研究核心2：表观遗传学核心 (Core导演：李晓刚;核心联合导演：肯尼斯·彼得森） 表观遗传学核心的目标是帮助PKD研究人员了解和调查 在PKD中转录和表观遗传调节被破坏的机制，并使用这种机制， 这些信息有助于制定具体有效的治疗策略。为此，表观遗传学核心将 为当地和扩展的PKD基地社区提供服务和试剂，以促进PKD的努力 研究人员在PKD科学的这一新的和令人兴奋的前沿作出尖端发现。芯会 利用肾脏研究所和KUMC表观遗传学中心的现有资源和能力 和干细胞生物学（由肯尼思彼得森博士执导），加上杰出的能力， KUMC提供的基因组测序设备和生物统计学和信息学共享资源。的 核心将帮助PKD研究人员识别和研究PKD相关基因的表观遗传修饰 并研究调节PKD相关信号通路的表观遗传机制。芯会 为研究人员提供各种快速周转服务，包括DNA甲基化分析，染色质 免疫沉淀（ChIP）、ChIP测序和实时定量PCR基因表达分析，以及 将提供关键表观遗传调节因子的敲除小鼠模型。这些服务和试剂将有助于 研究人员确定了PKD中被破坏的表观遗传信号通路， 为新疗法的开发提供了新的目标。核心还将帮助开展教育/咨询计划 帮助PKD研究人员更好地了解一般和PKD的表观遗传学机制， 技术培训，以帮助研究人员设计新的表观遗传学相关的研究项目。

Project Abstract Biomedical Research Core 2: Epigenetics Core (Core Director: Xiaogang Li; Core Co-Director: Kenneth Peterson) The goal of the Epigenetics Core is to help PKD researchers understand and investigate the mechanisms by which transcriptional and epigenetic regulation are disrupted in PKD, and to use this information to develop specific and effective therapeutic strategies. To this end, the Epigenetics Core will provide services and reagents to the local and extended base PKD community to facilitate the efforts of PKD investigators to make cutting-edge discoveries in this new and exciting frontier in PKD science. The Core will build upon existing resources and capabilities in the Kidney Institute, and the KUMC Center for Epigenetics and Stem Cell Biology (Directed by Dr. Kenneth Peterson), together with outstanding capabilities of the Genome Sequencing Facility and the Biostatistics and Informatics Shared Resource available at KUMC. The Core will help PKD researchers to identify and investigate epigenetic modifications on PKD-associated genes and to examine epigenetic mechanisms that regulate signaling pathways associated with PKD. The Core will provide a variety of rapid-turnaround services to researchers, including DNA methylation analysis, chromatin immunoprecipitation (ChIP), ChIP-sequencing, and real-time quantitative PCR gene expression analysis, and will provide knockout mouse models of key epigenetic regulators. These services and reagents will help investigators identify epigenetic signaling pathways disrupted in PKD and will facilitate the identification of targets for the development of new therapies. The Core will also help with an education/consultation program to help PKD researchers better understand epigenetics mechanisms in general and in PKD, and will provide technical training to help investigators design new epigenetics-related research projects.