Core B: Sample Preparation & Storage Core

样品制备和储存核心（核心B）的目标是标准化样品制备，集中 样本存储和分散，并优化P01内各项目之间的资源共享。 核心B服务将由四个项目的具体目标指导，这些目标是以下方面的科学驱动力： 这是P01为实现总体P01目标，核心B将建立一个基础设施， 样品制备的一致性、样品的有序储存以及检索储存样品的能力 以功能性的形式。核心B将通过提供和储存样本提供特殊的科学支持 使用标准化方法收集和制备。 核心B将由Dr. Daren L.指导。Knoell（PI）和小帕特里克罗斯博士（Co-I）并将通过 在俄亥俄州州立大学（OSU）接受过临床和技术支持培训。诺尔博士是金伯利教授 在药学系和戴维斯心肺研究所的成员与实质性的 人类和小鼠样本（包括人外周血）的采集和分析经验 单核细胞（PBMC）、小鼠和人支气管肺泡灌洗液（BAL）和细胞、小鼠尸检 用于器官收获，以及吞噬细胞（单核细胞、嗜中性粒细胞和巨噬细胞）和非吞噬细胞的培养。 吞噬细胞（AECI和AECII）。罗斯博士是胸外科主任和临床医学教授。 OSU医疗中心的外科手术。罗斯医生的专长是胸外科肿瘤学机器人手术 光动力疗法、肺减容和肺移植。罗斯博士和他的团队 具有进行支气管镜检查和处理/储存临床和研究标本的经验， 负责BAL程序，包括提供受试者招募和知情同意。 核心B将为小鼠细胞和组织的处理和储存提供集中支持， 支持安排人类受试者进行样本捐赠、获得人类受试者的知情同意，以及 人类样本的制备、传播和储存。中央支助将通过 （一）建立和维持一个基础设施和业务计划， 管理P01内每个项目的样本采集、制备和储存;以及ii） 原代人和小鼠细胞的制备和散布。核心B将与行政 通过每月会议，并通过就以下问题进行公开交流， 涉及细胞和流体的制备、储存和分配过程。

The goal of the Sample Preparation & Storage Core (Core B) is to standardize sample preparation, centralize sample storage and dispersion, and optimize the sharing of resources amongst each project within the P01. Core B services will be directed by the Specific Aims of the four projects that serve as the scientific drivers for this P01. To achieve the overall P01 objectives, Core B will establish an infrastructure that allows for the consistent preparation of samples, organized storage of samples, and the capacity to retrieve stored samples in a functional form. Core B will provide exceptional scientific support with the provision and storage of samples that are collected and prepared using standardization methodology. Core B will be directed by Dr. Daren L. Knoell (PI) and Dr. Patrick Ross Jr. (Co-I) and will be executed through trained clinical and technical support at The Ohio State University (OSU). Dr. Knoell is the Kimberly Professor in the Department of Pharmacy and a member of the Davis Heart and Lung Research Institute with substantial experience in the collection and analysis of human and mouse samples including human peripheral blood mononuclear cells (PBMC), mouse and human bronchoalveloar lavage (BAL) fluid and cells, mouse necropsy for organ harvest, and the culture of phagocytic (monocytes, neutrophils and macrophages) and non- phagocytic (AECI and AECII) cells. Dr. Ross is the chief of Thoracic Surgery and a Professor of Clinical Surgery at OSU Medical Center. Dr. Ross's expertise is in thoracic surgical oncology, robotic surgery, photodynamic therapy, lung volume reduction, and lung transplantation. Dr. Ross and his team have extensive experience in performing bronchoscopies and handling/storing clinical and research specimens and will be responsible for the BAL procedures, including the provision of subject recruitment and consent. Core B will provide centralized support for processing and storage of mouse cells and tissues and centralized support for the scheduling of human subjects for sample donation, consenting of human subjects, and the preparation, dissemination and storage of human samples. Centralized support will be achieved through the following Specific Aims: i) Establish and maintain an infrastructure and operational scheme that supports the management of sample collection, preparation, and storage for each individual project within the P01; and ii) Preparation and dissemination of primary human and mouse cells. Core B will interface with the Administrative Core (Core A) and each individual project through monthly meetings, and through open exchange with regard to the process of preparation, storage, and distribution of cells and fluids.