Cell Separation and Culture Core

摘要 细胞分离和培养核心的目标是提供许多人经常使用的服务 为调查人员提供资金，以便我们可以共享资源并降低成本。核心设施还提供 中心成员有一个促进科学合作的环境。在此应用程序中，我们将继续 细胞分离培养芯在肝细胞分离培养和肝移植中的应用 免疫细胞分选。此外，我们计划通过开发新技术来扩展我们的服务，以满足 中心成员日益增长的需求，如建立稳定表达Cas9至TO的肝细胞系 能够使用CASPR基因缺失系统以及分离肝脏祖细胞和培养iPS 支持不断增长的肝脏干细胞领域的细胞。为了实现这些目标，我们有以下几个方面 目标：1)我们将继续为广大客户提供优质、一致的服务 独立资助的研究人员通过共享资源和降低成本来提高研究效率。2) 我们将营造跨学科合作和发展的环境。3)我们将为以下人员提供培训 南加州大学和其他地方的研究人员正在研究细胞分离和分离技术。4)我们将开发新的 受资助的研究人员和初级教员将来可能用来推进他们的研究的服务 肝病的诊断、预防和治疗。

ABSTRACT The goal of the Cell Separation and Culture Core is to provide services that are frequently utilized by numerous funded investigators so that we may share resources and reduce costs. The core facility also provides the center members with an environment to promote scientific collaboration. In this application, we will continue the services from the Cell Separation and Culture Core for hepatocyte isolation and culturing and liver and immune cell sorting. In addition, we plan to expand our service by developing new techniques to meet the growing demand of the center members, such as establishing hepatocyte cell lines stably expressing Cas9 to enable the use of CASPR gene deletion system as well as isolation of liver progenitors and culturing of iPS cells to support the ever growing field of liver stem cells. To accomplish these goals, we have the following aims: 1) We will continue to provide high quality, consistent services needed by a large number of independently funded investigators to improve research efficiency by sharing resources and lowering costs. 2) We will foster an environment for interdisciplinary collaboration and growth. 3) We will provide training for investigators at USC and elsewhere in techniques for cell isolation and separation. 4) We will develop new services that the funded investigators and junior faculty will likely use in the future to advance their research in the diagnosis, prevention and treatment of livers diseases.