Mechanistic insight into the role of the BAM complex in E. coli

项目摘要 革兰氏阴性菌有一个外膜，为细胞提供营养和额外的保护。 此外，外膜含有蛋白质，使病原菌能够逃避宿主免疫 反应，并导致一些严重的传染病。尽管这些外部的重要性 膜蛋白（OMPs）的细胞生存和毒性，很少有人知道这些蛋白质是如何折叠的 并插入到外膜中。在大肠大肠杆菌，一个200 kDa的五组分复合物，称为BAM复合物 介导这些膜蛋白的生物合成，我的目标是使用功能测定， 结构生物学技术（X射线晶体学，电子显微镜），以表征每个 BAM复合物的组成部分以及底物OMP如何被识别。这些研究将有助于 揭示了革兰氏阴性菌外膜蛋白的生物合成机制，为研究外膜蛋白的生物合成提供了线索。 真核线粒体和叶绿体中外膜蛋白的生物发生。

Project summary Gram-negative bacteria have an outer membrane which provides nutrients and added protection for the cell. Additionally, the outer membrane contains proteins that enable pathogenic bacteria to evade host immune responses and cause a number of serious infectious diseases. Despite the importance of these outer membrane proteins (OMPs) for cell survival and virulence, little is known about how these proteins are folded and inserted into the outer membrane. In E. coli, a 200 kDa five component complex called the BAM complex mediates the biogenesis of these membrane proteins and it is my goal is to use functional assays and structural biology techniques (X-ray crystallography, electron microscopy) to characterize the role that each component of the BAM complex serves and how substrate OMPs are recognized. These studies will assist in revealing the mechanism for the biogenesis of OMPs in Gram-negative bacteria and will offer clues to the biogenesis of OMPs in eukaryotic mitochondria and chloroplasts.