Center for Advanced Microscopy Shared Resource

摘要-高级显微镜中心 高级显微镜中心（CAM）提供培训、访问、支持和专业知识， 癌症中心（LCC）成员成功地利用先进的显微镜技术和仪器， 他们的研究它被西北大学（NU）的研究人员大量使用，有104名LCC成员使用 设施去年。我们的设施是开放的24小时/每周7天，并提供访问的状态， 先进的光学和电子显微镜（EM）仪器，辅助设备和试剂，允许成像， 在一定范围的空间尺度上进行。我们不断扩大的仪器产品组合， CAM人员的专业知识使用户能够对单个分子、大分子网络、活细胞、组织 切片、整体器官和具有明场、荧光和发光的模式生物。我们支持 先进的成像技术，如FRET，FRAP，FLIP，TIRF，光转换和免疫金， 铂金复制品EM。该基金积极地从一个国家筹集资金，并取得了很大的成功。 LCC的各种来源，以保持在新的成像技术的领导地位。例如，CAM是 尼康在美国只有三个成像中心，让我们的用户可以测试最新的技术， 尼康CAM还提供工作站、一套软件和专家支持，包括最近聘请的Image 分析专员，促进成像数据的传输、存储、管理和分析。该设施还 提供了广泛的教育计划，包括课程，研讨会，期刊俱乐部，和实践 工作坊.

ABSTRACT – CENTER FOR ADVANCED MICROSCOPY The Center for Advanced Microscopy (CAM) provides training, access, support and expertise that enable Lurie Cancer Center (LCC) members to successfully utilize advanced microscopy techniques and instrumentation in their studies. It is heavily used by Northwestern University (NU) investigators, with 104 LCC members using the facility last year. Our facility is open 24 hours/7 days a week and provides access to a wide array of state of the art light and electron microscopy (EM) instrumentation, auxiliary equipment, and reagents, permitting imaging to be performed across a range of spatial scales. Our continuously expanding portfolio of instruments and the expertise of CAM staff enable users to image single molecules, macromolecular networks, live cells, tissue sections, whole organs and model organisms with brightfield, fluorescence and luminescence. We support advanced imaging techniques such as FRET, FRAP, FLIP, TIRF, photo-conversion, and immuno-gold and platinum replica EM. The facility has aggressively, and with a high degree of success, pursued funding from a variety of sources to maintain the LCC’s leadership in novel imaging technologies. For example, CAM is one of only three Nikon Imaging Centers in the United States, allowing our users to test the latest technologies from Nikon. CAM also provides workstations, a suite of software, and expert support, including a recently hired Image Analysis Specialist, to facilitate imaging data transfer, storage, management and analysis. The facility also provides a wide array of educational programs, including courses, seminars, journal clubs, and hands-on workshops.