Request for Support to Purchase Automated Microscope

补充摘要 本申请要求与NIGMS研究员Rajat博士合作， Rohatgi，Olympus IX 83落射荧光显微镜。这一工具将有助于推进我的目标， NIGMS R01奖（R01 GM115968），使我们能够研究有缺陷的蛋白质的命运， 核糖体相关质量控制（亲本R01的目标2）。奥林巴斯IX83是一款全电动、自动化的 倒置显微镜系统，包括基于激光的自动对焦（用于高通量成像），载物台顶部 用于环境控制、光漂白后荧光恢复（FRAP）和迭代的培养箱 反卷积能力。具有这些能力的仪器目前还没有成本效益高的 这是我的研究计划或Rajat Rohatgi博士的研究计划的基础。斯坦福大学生物化学系 将支持这两个仪器服务的资金和空间来容纳显微镜系统购买。在 总之，这种显微镜将加速我们实验室的NIGMS资助的研究项目， 这些都是斯坦福大学生物化学系其他NIGMS资助的研究人员的研究成果。 ​​

Supplement Summary This application requests funds for the joint purchase, in partnership with NIGMS investigator Dr. Rajat Rohatgi, of an Olympus IX83 epifluorescence microscope. This instrument will help advance the goals of my NIGMS R01 award (R01 GM115968) by allowing us to study the fate of defective proteins which have escape Ribosome-associated Quality Control (Aim 2 of parent R01). The Olympus IX83 is a fully-motorized, automated inverted microscope system that includes laser-based autofocus (for high throughput imaging), a stage top incubator for environmental control, fluorescence recovery after photobleaching (FRAP) and iterative deconvolution capabilities. An instrument with these capabilities is not presently available on a cost-effective basis for either my research program or that of Dr. Rajat Rohatgi. The Biochemistry Department at Stanford will support this purchase with both funds for instrument service and space to house the microscope system. In summary, this microscope will accelerate the NIGMS-funded research projects in our laboratories and more broadly those of other NIGMS-funded investigators in the Department of Biochemistry at Stanford. ​​