Cytometry Core

项目摘要：细胞计数核心 Cytometry Core的主要目标是通过提供 成本有效的集中资源，用于使用基于抗体的细胞预富集，随后的流动 基于细胞计数的分析和纯化，用于2D和3D成像的薄切片和全/厚冷冻切片，以及 定量成像细胞术。核心将提供1）8参数流式细胞仪，这是主力 用于所有三个项目中提议的大多数流式细胞术实验的分析仪器，2）a 用于定量成像细胞仪的激光扫描细胞仪（LaSC）的集中式、经济高效的资源 在所有三个项目中提出的分析，3）制备厚的低温恒温器组织切片和3D共焦成像，4）a 在设计和分析复杂的流式细胞仪方面提供专家建议和咨询的资源 和LaSC实验，包括用于流式细胞术分析和iCys细胞计数的FlowJo软件许可证 用于LaSC分析的分析软件（CompuCyte），和5）用于细胞玫瑰花结的磁体和试剂， 用于在随后的流式细胞术之前预富集感兴趣的细胞的基于磁性颗粒的策略 基于分析和/或排序。通过为基于抗体的细胞免疫提供具有成本效益的集中资源， 预富集和随后的基于细胞计量术（流式细胞术和LaSC）的分析和纯化， 细胞计数核心将有助于进一步了解控制功能的途径的计划目标 骨髓、脐带血和外周来源的细胞。

Project Summary: Cytometry core The key objective of the Cytometry Core is to enhance the overall productivity of the Program by providing a cost-effective, centralized resource for the use of antibody based cellular pre-enrichment, subsequent flow cytometry based analysis and purification, thin and whole/thick frozen sections for 2D and 3D imaging, and quantitative imaging cytometry. The core will provide 1) the 8 parameter flow cytometer that is the workhorse analytical instrument for the majority of flow cytometry experiments proposed in all three projects, 2) a centralized, cost-effective resource for the laser scanning cytometry (LaSC) for quantitative imaging cytometry analysis proposed in all three projects, 3) preparation of thick cryostat tissue sections and 3D confocal imaging, 4) a resource for expert advice and consultation in the design and analysis of complex, multicolor flow cytometry and LaSC experiments including FlowJo software licenses for flow cytometry analysis and iCys cytometric analysis software (CompuCyte) for LaSC analysis, and 5) magnets and reagents for cellular rosetting and magnetic-particle based strategies for pre-enrichment of cells of interest prior to subsequent flow cytometry based analysis and/or sorting. By providing a cost-effective, centralized resource for antibody based cellular pre-enrichment and subsequent cytometry (flow cytometry and LaSC) based analysis and purification, the Cytometry core will help further the Programs goals of understanding the pathways which control the function of transfused bone marrow, cord blood, and peripherally derived cells.