Shared Resource: Proteomics and Metabolomics

蛋白质组学和代谢组学共享资源 项目总结/摘要 蛋白质组学和代谢组学共享资源（PMSR）由弗雷德哈钦森癌症研究 中心（FHCRC）的蛋白质组学/代谢组学实验室和华盛顿大学南湖分校 UW-SLU实验室。PMSR的蛋白质组学部分成立于2002年11月，其目标是 提供具有成本效益的高效液相色谱和质谱为基础的蛋白质组学 癌症联盟（Cancer Consortium）成员。蛋白质组学资源的使命是提供高 及时为小规模和大规模的定性和定量蛋白质组学分析提供优质服务， 蛋白质修饰表征和靶向蛋白质组学分析。这些服务从 在模型系统中小规模鉴定蛋白质复合物和鉴定高度纯化的 血清样品和磷酸化蛋白质组学中的大规模生物标志物发现/验证实验 动物器官分析PMSR在2008年获得了优秀的绩效评估。 2010年，PMSR扩大到包括代谢组学研究和相关资源， 招募丹尼尔·拉夫特里博士一个公认的领导者在代谢组学研究，以FHCRC和 美国华盛顿大学沿着上面提到的蛋白质组学服务， 提供水性代谢物和脂质的非靶向分析（脂质组学），代谢物的靶向分析 从通过质谱和核磁共振光谱的许多代谢途径， 和同位素示踪能力。PMSR也在迅速发展质谱磷脂 分析.该资源致力于开发和实施新的蛋白质组学和代谢组学工具 为了揭示影响癌症生物学的分子细节， 诊断和临床试验。 蛋白质组学和代谢组学资源的科学运作分为FHCRC和UW- 医学网站Gafken博士负责FHCRC的所有业务，Raftery博士负责UW的业务。Drs. Gafken和Raftery每月举行一次会议，以协调活动，如果需要，可以更频繁地进行特定活动。 项目所有基于蛋白质组学的项目都在FHCRC进行，而基于代谢组学的项目则在 在两个网站之间划分。代谢组学项目，其中已建立，常规检测是可用的 在FHCRC进行，而那些非常规的代谢组学项目，需要大量的 开发在UW进行。随着新的代谢组学检测方法在华盛顿大学的研究中心得到开发和完善， FHCRC的工作人员将接受这些协议的培训，以使其作为一项服务更广泛地提供。所有 NMR能力和NMR相关项目将在UW-SLU进行。

Proteomics and Metabolomics Shared Resource Project Summary/Abstract The Proteomics and Metabolomics Shared Resource (PMSR) consists of a Fred Hutchinson Cancer Research Center (FHCRC)-based Proteomics/Metabolomics laboratory and a University of Washington-South Lake Union (UW-SLU) laboratory. The Proteomics section of the PMSR was formed in November 2002 with the goal of providing cost-effective high performance liquid chromatography and mass spectrometry-based proteomics services to Cancer Consortium (Consortium) members. The Proteomics resource's mission is to provide high quality service in a timely manner for small- and large-scale qualitative and quantitative proteomics analyses, protein modification characterization, and targeted proteomic analyses. These services have evolved from small-scale identification of protein complexes in model systems and identifying modifications on highly purified proteins to large-scale biomarker discovery/validation experiments in serum samples and phosphoproteomics analysis of animal organs. The PMSR received an outstanding merit assessment in 2008. In 2010, the PMSR was expanded to include metabolomics research and related resources through the joint recruitment of Daniel Raftery, Ph.D., a recognized leader in metabolomics research, to FHCRC and the University of Washington (UW). Along with the proteomics services mentioned above, the resource now provides untargeted profiling of aqueous metabolites and lipids (lipidomics), targeted profiling of metabolites from numerous metabolic pathways via mass spectrometry and nuclear magnetic resonance spectroscopy, and isotope tracer capabilities. The PMSR is also rapidly developing mass spectrometry phospholipid analysis. The resource is committed to developing and implementing new proteomics and metabolomics tools in order to uncover the molecular details that influence cancer biology and to support development of diagnostic and clinical tests. Scientific operations of the Proteomics and Metabolomics resource are divided between FHCRC and UW- medicine sites. Dr. Gafken oversees all operations at FHCRC and Dr. Raftery oversees those at UW. Drs. Gafken and Raftery meet on a monthly to coordinate activities, or more frequently if needed for specific projects. All proteomics-based projects are performed at FHCRC while metabolomics-based projects are divided between the two sites. Metabolomics projects for which established, routine assays are available are performed at FHCRC while those metabolomics projects that are non-routine and require significant development are performed at UW. As new metabolomics assays are developed and refined at the UW site, FHCRC-based staff will be trained on these protocols to make them more widely available as a service. All NMR capabilities and NMR-related projects will be conducted at UW-SLU.