Omics and Bioinformatics Facility Core

摘要(OMICS和生物信息学核心) 下一代测序的应用，靶向组学分析的方案，以及数量和广度 所有可公开获得的组学数据集都在继续快速扩张。因此，很难做到这一点 环境健康科学家随时了解所有最新的技术、分析方法和可用的 与他们的研究相关的数据。OMICS和生物信息学核心(OBIC)的目标是提供 为密歇根生命环境中心的调查人员提供全面和创新的支持 暴露和疾病(M-LEEAD)，用于研究设计、样本准备、分析、解释和 整合广泛的以组学为基础的研究，并就现有的机会对他们进行教育。奥比克 简化了组学研究的整个过程，使中心研究人员能够致力于自己的更多工作 用于回答环境健康科学难题的资源。寿命周期环境暴露 可能通过各种分子机制增加日后患病的风险，包括表观遗传、 遗传、转录和代谢组学机制。我们提供专业知识和技术的技术 分析包括焦磷酸测序和测序、RNA-seq、基因表达和DNA甲基化 微阵列，microRNA表达，人甲基化450珠芯片，亚硫酸氢盐还原代表 测序(RRBS)和MeDIP序列，以及芯片序列。欧比克的目标将通过四个阶段实现 明确的目标。首先，我们将为M-Leead调查人员提供设计和实施 广泛的组学和基因靶标研究(例如基因组、表观基因组、蛋白质组、代谢组)和 制定协议，包括技术平台的选择，以及样品的采集和准备。 第二，我们将为M-Leead调查人员提供广泛的生物信息学分析服务， 包括标准或定制分析、对数据管理和可视化的支持以及路径/网络 分析。第三，OBIC通过开发新的生物信息学方法和工具来支持社区 和新技术的协议与技术进步保持同步。最后，欧比克教育 与环境最相关的组学分析和生物信息学方法的中心教职员工和学生 健康科学研究，并将环境科学家与组学和 生物信息学。M-Leead调查人员受益于免费和/或降低成本的服务。我们的服务包括 在赠款和手稿编写方面的援助，关于使用工具和资源的个人培训，以及 面向M-Leead调查人员的生物信息学/Omics研讨会。

ABSTRACT (Omics and Bioinformatics Core) Applications of next generation sequencing, protocols for targeted omics assays, and the number and breadth of publicly-available omics datasets all continue to expand at a rapid pace. As such, it is difficult for environmental health scientists to stay up-to-date on all of the technologies, analysis methods, and available data relevant to their research. The objective of the Omics & BioInformatics Core (OBIC) is to provide comprehensive and innovative support to investigators in the Michigan Center on Lifestage Environmental Exposures and Disease (M-LEEaD) for the study design, sample preparation, analysis, interpretation, and integration of a broad range of omics-based studies, and to educate them on available opportunities. OBIC streamlines the entire process of an omics study, allowing center investigators to dedicate more of their own resources to answering difficult environmental health science questions. Lifestage environmental exposures may increase the risk of later disease through a variety of molecular mechanisms, including epigenetic, genetic, transcriptomic, and metabolomics mechanisms. Technologies for which we provide expertise and analysis include pyrosequencing and Sequenom, RNA-seq, gene expression and DNA methylation microarrays, microRNA expression, HumanMethylation450 BeadChip, reduced representation bisulfite sequencing (RRBS) and MeDIP-seq, and ChIP-seq. The OBIC objective will be accomplished through four specific aims. First, we will provide M-LEEaD investigators expertise in the design and implementation of a broad range of omics and gene target studies (e.g. genomic, epigenomic, proteomic, metabolomic) and development of protocols, including technology platform choice, and sample collection and preparation. Second, we will provide M-LEEaD investigators with a broad-range of bioinformatics analysis services, including standard or custom analyses, support for data management and visualization, and pathway/network analyses. Third, OBIC supports the community through development of novel bioinformatics methods and tools and protocols for novel technologies staying apace with technological advancements. Finally, OBIC educates center faculty, staff, and students on omics assays and bioinformatics methods most relevant to environmental health sciences research, and connects environmental scientists with potential collaborators in omics and bioinformatics. M-LEEaD investigators benefit from free and/or reduced cost services. Our services include assistance in grants and manuscript preparation, individual training on the use of tools and resources, and Bioinformatics/Omics workshops targeted to M-LEEaD investigators.