Omics and Bioinformatics Facility Core

组学和生物信息学设施核心

• 批准号: 9058300
• 负责人: Maureen Agnes Sartor
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9058300
• 关键词: Address; Alternative Splicing; Bioinformatics; Biological; Biological Assay; Cell Count; ChIP-seq; Chromatin; Communities; Complex; Core Facility; Custom; DNA Methylation; Data; Data Set; Databases; Development; Disease; Educational workshop; Environmental Exposure; Environmental Health; Epigenetic Process; Faculty; Funding; Gene Expression; Gene Targeting; Generations; Genes; Genetic; Genetic Transcription; Genome; Genomic Segment; Genomics; Grant; Individual; Life; Link; Manuscripts; Methodology; Methods; Michigan; MicroRNAs; Mission; Molecular; Molecular Conformation; Paraffin Embedding; Pathway Analysis; Pathway interactions; Phenotype; Policies; Preparation; Process; Proteins; Proteomics; Protocols documentation; Publications; Recommendation; Research; Research Design; Research Personnel; Resources; Risk; Sampling; Scientist; Services; Signal Pathway; Staging; Students; Techniques; Technology; Training; Transcript; Untranslated RNA; base; bead chip; bisulfite sequencing; cost; data management; data sharing; data visualization; design; epigenomics; genetic variant; genome-wide; genome-wide analysis; health science research; high throughput technology; histone modification; innovation; meetings; member; metabolomics; new technology; next generation sequencing; novel; protein metabolite; protocol development; pyrosequencing; ranpirnase; sample collection; tool; toxicant; transcription factor; transcriptome sequencing; transcriptomics

ABSTRACT (Omics and Bioinformatics Core) Applications of next generation sequencing, protocols for targeted omics assays, and the number and breadth of publicly-available omics datasets all continue to expand at a rapid pace. As such, it is difficult for environmental health scientists to stay up-to-date on all of the technologies, analysis methods, and available data relevant to their research. The objective of the Omics & BioInformatics Core (OBIC) is to provide comprehensive and innovative support to investigators in the Michigan Center on Lifestage Environmental Exposures and Disease (M-LEEaD) for the study design, sample preparation, analysis, interpretation, and integration of a broad range of omics-based studies, and to educate them on available opportunities. OBIC streamlines the entire process of an omics study, allowing center investigators to dedicate more of their own resources to answering difficult environmental health science questions. Lifestage environmental exposures may increase the risk of later disease through a variety of molecular mechanisms, including epigenetic, genetic, transcriptomic, and metabolomics mechanisms. Technologies for which we provide expertise and analysis include pyrosequencing and Sequenom, RNA-seq, gene expression and DNA methylation microarrays, microRNA expression, HumanMethylation450 BeadChip, reduced representation bisulfite sequencing (RRBS) and MeDIP-seq, and ChIP-seq. The OBIC objective will be accomplished through four specific aims. First, we will provide M-LEEaD investigators expertise in the design and implementation of a broad range of omics and gene target studies (e.g. genomic, epigenomic, proteomic, metabolomic) and development of protocols, including technology platform choice, and sample collection and preparation. Second, we will provide M-LEEaD investigators with a broad-range of bioinformatics analysis services, including standard or custom analyses, support for data management and visualization, and pathway/network analyses. Third, OBIC supports the community through development of novel bioinformatics methods and tools and protocols for novel technologies staying apace with technological advancements. Finally, OBIC educates center faculty, staff, and students on omics assays and bioinformatics methods most relevant to environmental health sciences research, and connects environmental scientists with potential collaborators in omics and bioinformatics. M-LEEaD investigators benefit from free and/or reduced cost services. Our services include assistance in grants and manuscript preparation, individual training on the use of tools and resources, and Bioinformatics/Omics workshops targeted to M-LEEaD investigators.

摘要（组学和生物信息学核心） 下一代测序的应用、靶向组学分析的方案以及数量和广度 公开可用的组学数据集都在继续快速扩展。因此，很难 环境健康科学家了解所有技术、分析方法和可用的最新信息 与他们的研究相关的数据。组学和生物信息学核心 (OBIC) 的目标是提供 为密歇根生命阶段环境中心的研究人员提供全面和创新的支持 暴露与疾病 (M-LEEaD) 用于研究设计、样品制备、分析、解释和 整合广泛的基于组学的研究，并教育他们现有的机会。奥比克 简化组学研究的整个过程，使中心研究人员能够投入更多的精力 回答困难的环境健康科学问题的资源。生命阶段环境暴露 可能通过多种分子机制增加后期疾病的风险，包括表观遗传、 遗传、转录组和代谢组学机制。我们提供专业知识和技术的技术 分析包括焦磷酸测序和 Sequenom、RNA-seq、基因表达和 DNA 甲基化 微阵列、microRNA 表达、HumanMmethylation450 BeadChip、还原代表性亚硫酸氢盐 测序 (RRBS) 和 MeDIP-seq 和 ChIP-seq。 OBIC 的目标将通过四个方面来实现 具体目标。首先，我们将为 M-LEEaD 研究人员提供设计和实施的专业知识 广泛的组学和基因靶标研究（例如基因组学、表观基因组学、蛋白质组学、代谢组学）和 协议的开发，包括技术平台的选择以及样品的收集和制备。 其次，我们将为 M-LEEaD 研究人员提供广泛的生物信息学分析服务， 包括标准或自定义分析、数据管理和可视化支持以及路径/网络 分析。第三，OBIC 通过开发新型生物信息学方法和工具来支持社区 与技术进步保持同步的新技术协议。最后，OBIC教育 中心教职员工和学生学习与环境最相关的组学分析和生物信息学方法 健康科学研究，并将环境科学家与组学和潜在合作者联系起来 生物信息学。 M-LEEEaD 调查员受益于免费和/或减价服务。我们的服务包括 协助赠款和手稿准备、有关工具和资源使用的个人培训，以及 针对 M-LEEaD 研究人员的生物信息学/组学研讨会。