Miniaturized head-mounted device for pan-cortical electro-optical activity monitoring

项目总结 本申请是为了响应生物工程研究基金的R01机制而提交的。这 应用程序提出开发一种无缝集成广域的小型化头盔设备 光学成像和皮层脑电(ECoG)记录，以实现整体的多模式活动映射 自由行为时的大脑皮层。这项建议建立在我们实验室的技术发展基础上--透明 聚合物头骨，提供光学接触到大约80万到100万个神经元，横跨90平方毫米的 小鼠背侧皮质的表面。在目标1中，我们将开发头盔成像系统，用于 自由活动和行为的小鼠整个背侧皮质的中尺度活动图谱。在AIM 2中，我们将 集成多层喷墨打印皮层脑电图仪的工程透明聚合物头骨 允许同时进行光学成像和皮质表面场电位记录的电极阵列。我们会 工程师将定制接口电子设备集成到AIM 1中开发的头盔设备中，以实现 在自由活动的小鼠中同时进行皮层脑电记录和皮质广泛成像。在目标3中，我们将利用 在AIMS 1和AIMS 2中开发的设备，用于研究新对象期间皮质活动模式的动力学 识别和探索任务。这些发展将从根本上使新的实验类型成为可能 在老鼠身上进行的研究，揭示了对构成感官知觉基础的神经元计算的新见解， 行动和认知过程。

PROJECT SUMMARY This application is submitted in response to the Bioengineering Research Grant’s R01 mechanism. This application proposes to develop a miniaturized head-mounted device that seamlessly integrate wide-field optical imaging and electrocorticogram (ECoG) recordings to achieve multimodal activity mapping of the whole cortex during free behavior. The proposal builds on technological developments in our lab – transparent polymer skulls that provide optical access to an estimated 800,000 to 1,000,000 neurons across 90 mm2 of the surface of the dorsal cortex of the mouse. In AIM 1 we will develop head-mounted imaging systems for mesoscale activity mapping of the whole dorsal cortex in freely moving and behaving mice. In AIM 2, we will engineer transparent polymer skulls integrated with multilayer inkjet-printed electrocorticogram (ECoG) electrode arrays that allows simultaneous optical imaging and cortical surface field potential recordings. We will engineer custom interface electronics integrated into the head-mounted device developed in AIM 1 to enable simultaneous ECoG recordings and cortex wide imaging in freely moving mice. In AIM 3 we will utilize the devices developed in Aims 1 and 2, to study dynamics of cortical activity patterns during novel object recognition and exploration tasks. These developments will enable fundamentally new kinds of experimental studies in the mouse and reveal new insights into the neuronal computations that underlie sensory perception, action and cognitive processes.