Eye to Brain: Eye Biomarkers and Cognition

项目摘要 我们研究的目的是鉴定对诊断和潜在预后有用的眼部生物标志物， 阿尔茨海默氏病（AD），并建立与认知的关系。我们试图确定这种关系， 一个已经建立的眼病患者队列（维生素生物标志物研究，或VBS），其中 已经在眼睛的玻璃体液中鉴定了蛋白质生物标志物， 简易精神状态检查（MMSE）功能评分。需要敏感和具体的 可以在早期阶段检测AD的诊断测试。我们假设，Aβ和Tau的眼部水平， 眼的房水以及通过光学成像的神经变性的视网膜生物标志物， 相干断层扫描（OCT）与认知评估相结合，可提供以下预测价值： 早期AD的检测在VBS中，我们发现在VBS中β淀粉样蛋白（Aβ40，Aβ42）和总Tau（tTau）的水平与VBS中的β淀粉样蛋白（Aβ40，Aβ42）和总Tau（tTau）的水平相关。 玻璃体液与简易精神状态检查获得的认知功能评分相关 （MMSE）。从相同的患者队列中，我们试图建立相同的蛋白质的存在， 眼的房水（其在临床上比玻璃体液更易接近），获得OCT图像以 寻找视网膜生物标志物，并重复MMSE测试，以纵向观察认知，并将所有眼睛 生物标志物与认知此外，我们还试图验证房水和玻璃体中的蛋白质水平， 尸体的眼神如果蛋白质和视网膜生物标志物与认知的关系可以 我们对眼睛在AD中的作用的认识将大大增加，它将建立眼睛作为 在我们诊断AD的过程中扮演着重要的角色

PROJECT SUMMARY The goal of our research is to identify ocular biomarkers that have diagnostic and potentially prognostic utility in Alzheimer's Disease (AD), and establish a relationship to cognition. We seek to determine this relationship in an already established cohort of patients (the Vitreous Biomarkers Study, or VBS) with eye disease where protein biomarkers have been identified in the vitreous humor of the eye and found to be correlated to cognitive function scores on mini-mental status examination (MMSE). There is a need for sensitive and specific diagnostic tests that can detect AD in the early stages. We hypothesize that ocular levels of Aβ and Tau in the aqueous humor of the eye as well as retinal biomarkers of neurodegeneration imaged by Optical Coherence Tomography (OCT), combined with cognitive assessments, may offer a predictive value for detection of early AD. In the VBS, we found that levels of beta amyloid (Aβ40, Aβ42), and total Tau (tTau) in the vitreous humor are correlated with cognitive function scores obtained by mini-mental status examination (MMSE). Drawing from the same patient cohort, we seek to establish the presence of the same proteins in the aqueous humor of the eye (which is more clinically accessible than the vitreous humor), obtain OCT images to look for retinal biomarkers, and repeat MMSE testing to look longitudinally at cognition, and correlate all eye biomarkers with cognition. Additionally, we seek to validate the protein levels in the aqueous and vitreous humor of eyes from postmortem subjects. If a relationship of protein and retinal biomarkers to cognition can be shown, our knowledge of the eye's role in AD will be increased substantially and it will establish the eye as potentially playing a central role in how we diagnose AD.