Botanical Core

植物学核心：摘要/总结 拟议的密西西比大学（UM）植物膳食植物核心的总体目的 补充剂研究中心（BDSRC）是为了确保产品的完整性和推进化学研究， 基于螺旋藻的产品Immulina™，使用基于生物测定和基于化学的方法的组合。 隶属于拟议的UM BDSRC的研究人员已经生成了支持可重复性和 Immulina™用于增强宿主反应的可能促进恢复力的机制上合理的作用 预防呼吸道病毒感染，如流感，导致959，000名患者住院治疗， 仅在美国就有79,400人死亡。 植物学核心将由Amar Chittiboyina博士领导，他是一位具有广泛研究的药物化学家 在天然产物化学以及标准化方法方面的经验。 植物核心的具体目标是： 1.确保对节旋藻（螺旋藻）进行明确的识别并开展额外的安全性测试 将用于生产足够量的Immulina™的Platensis原料， 建议的研究项目; 2.开发并验证用于定量Toll样受体2依赖性的选择性体外生物测定法 由Immulina™中的Braun型脂蛋白表现出的活性; 3.通过建立基于化学的认证，推进Immulina™的化学研究， 标准化方法，免疫抑制物质的表征和详细的结构 分析主动免疫增强型Braun型脂蛋白;和 4.进行额外的稳定性研究，提供足量的充分表征的安全 ImmulinaTM用于拟议的UM BDSRC研究项目，并探索合作 与其他碳单位合作的机会。 预期成果包括（1）实现更高水平的产品完整性和质量保证， Immulina™用于拟议的UM BDSRC项目;（2）设计改进的生物标准化 仅选择性检测TLR 2激动剂如Immulina™中的Braun型脂蛋白的方法;（3） 提供基于化学的认证和标准化方法，以补充生物测定- 的标准化方法，并提供一个强大的系统，以充分表征Immulina™产品材料; 和（4）提供足够数量的标准化Immulina™与其他研究人员密切合作， UM BDSRC和合作与其他单位的碳对ImmulinaTM未来的疗效研究。

BOTANICAL CORE: ABSTRACT/SUMMARY The overall purpose of the Botanical Core of the proposed University of Mississippi (UM) Botanical Dietary Supplements Research Center (BDSRC) is to ensure product integrity and advance chemistry research on the Spirulina-based product, Immulina™, using a combination of bioassay- and chemical-based approaches. Researchers affiliated with the proposed UM BDSRC have generated evidence that supports reproducible and mechanistically plausible effects of Immulina™ for enhancing host responses that may promote resilience against respiratory viral infections such as influenza which resulted in 959,000 patient hospitalizations and 79,400 deaths in United States alone. The Botanical Core will be headed by Amar Chittiboyina, PhD, a medicinal chemist with extensive research experience in natural product chemistry as well as standardization methodologies. The specific aims of the Botanical Core are to: 1. Ensure the unambiguous identification and develop additional safety testing on Arthrospira (Spirulina) platensis raw material that will be used in the production of sufficient quantities of Immulina™ for the proposed research projects; 2. Develop and validate a selective in vitro bioassay for quantitation of the toll-like receptor 2-dependent activity exhibited by the Braun-type lipoproteins in Immulina™; 3. Advance chemistry research on Immulina™ by establishing chemical-based authentication and standardization approaches, characterization of immune-inhibitory substances and detailed structural analysis of the active immune-enhancing Braun-type lipoproteins; and 4. Perform additional stability studies, provide sufficient quantities of well-characterized and safe ImmulinaTM for use in the proposed UM BDSRC research projects, and explore collaborative opportunities with other CARBON units. Expected outcomes include (1) achieving a higher level of product integrity and quality assurance of Immulina™ used in the proposed UM BDSRC projects; (2) design of an improved biological standardization method that will selectively detect only TLR2 agonists such as the Braun-type lipoproteins in Immulina™; (3) providing chemical-based authentication and standardization methods that will complement the bioassay- based standardization method and provide a robust system to fully characterize Immulina™ product material; and (4) to provide sufficient quantities of standardized Immulina™ working closely with other researchers from the UM BDSRC and collaborating with other units of CARBON on future efficacy studies of ImmulinaTM.